Skórski T, Kawalec M, Kawiak J
Medical Centre of Postgraduate Education, Warsaw, Poland.
Transplantation. 1991 Apr;51(4):843-7. doi: 10.1097/00007890-199104000-00020.
BALB/cxDBA/2Wf F1 (CD2F1) mice were lethally irradiated and reconstituted with syngeneic bone marrow cells untreated or treated with 75 micrograms/ml of mafosfamide. One day after bone marrow transplantation some groups of mice were injected with syngeneic splenocytes, peripheral blood leukocytes, or thymocytes. Seven days after marrow grafting the anti-L1210 leukemia immunization of mice, consisting of four i.p. injections of 10(6) L1210-Maf cells (L1210 cells treated in vitro with mafosfamide for inhibition of their growth in vivo), was started. Strong resistance against leukemia could be obtained only in mice receiving splenocytes or peripheral blood leukocytes, not in mice injected with thymocytes or in those not receiving any cells. In vitro elimination of various subpopulations from among the splenocytes before their injection into the mice made it possible to deduce which are necessary for early induction of antitumor resistance after bone marrow transplantation in mice. These cells are: Thy 1.2-, Ig-, AsGM 1-, Mac 1+, 1-Ad+/-, are adherent and nonsusceptible to carrageenan toxicity.
将BALB/cxDBA/2Wf F1（CD2F1）小鼠进行致死性照射，然后用未经处理或用75微克/毫升马磷酰胺处理的同基因骨髓细胞进行重建。骨髓移植后一天，部分小鼠组被注射同基因脾细胞、外周血白细胞或胸腺细胞。骨髓移植七天后，开始对小鼠进行抗L1210白血病免疫，包括腹腔注射四次10⁶个L1210-Maf细胞（L1210细胞在体外经马磷酰胺处理以抑制其在体内生长）。只有接受脾细胞或外周血白细胞的小鼠能获得对白血病的强抵抗力，注射胸腺细胞的小鼠或未接受任何细胞的小鼠则不能。在将脾细胞注射到小鼠体内之前，对其各种亚群进行体外清除，从而有可能推断出哪些细胞对于小鼠骨髓移植后早期诱导抗肿瘤抵抗力是必需的。这些细胞是：Thy 1.2⁻、Ig⁻、AsGM 1⁻、Mac 1⁺、1-Ad⁺/⁻，具有黏附性且对卡拉胶毒性不敏感。
