Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University, Beijing, 100084, China.
Chem Asian J. 2010 Apr 1;5(4):919-24. doi: 10.1002/asia.200900490.
In this study, we describe the use of the combination of eletrografting poly(N-mercaptoethyl acrylamide) and Au nanoparticles in the construction of high-performance biosensors. The poly(N-mercaptoethyl acrylamide) was electrografted onto the glassy carbon electrode surface, which provided a strongly adhering primer film for the stable attachment of Au nanoparticles and horseradish peroxidase (HRP) enzymes. The performances of the biosensors based on the HRP immobilized in the Au/poly(N-mercaptoethyl acrylamide) composite film were investigated. A couple of redox peaks were obtained, indicating that the Au nanoparticles could facilitate the direct-electron transfer between HRP and the underlying electrode. The biosensor showed an excellent electrocatalytic activity toward the reduction of hydrogen oxide and long-term stability, owing to the stable electrografted film and biocompatible Au nanoparticles. Our results demonstrate that the combination of electrografting and Au nanoparticles provides a promising platform for the immobilization of biomolecules and analysis of redox enzymes for their sensing applications.
在这项研究中，我们描述了将聚（N-巯基乙基丙烯酰胺）和金纳米粒子的组合用于构建高性能生物传感器的用途。聚（N-巯基乙基丙烯酰胺）被电化学接枝到玻碳电极表面，为金纳米粒子和辣根过氧化物酶（HRP）酶的稳定附着提供了一个牢固的底漆膜。基于固定在 Au/聚（N-巯基乙基丙烯酰胺）复合膜中的 HRP 的生物传感器的性能进行了研究。获得了一对氧化还原峰，表明金纳米粒子可以促进 HRP 和底层电极之间的直接电子转移。由于稳定的电化学接枝膜和生物相容性的金纳米粒子，该生物传感器对氢氧化物的还原表现出优异的电催化活性和长期稳定性。我们的结果表明，电化学接枝和金纳米粒子的组合为生物分子的固定化和氧化还原酶的分析提供了一个有前途的平台，用于它们的传感应用。
