Laboratory of Inorganic Materials Chemistry (CMI), The University of Namur (FUNDP), 61 Rue de Bruxelles, B-5000 Namur, Belgium.
Langmuir. 2010 May 4;26(9):6568-75. doi: 10.1021/la9039286.
The encapsulation of living plant cells into materials could offer the possibility to develop new green biochemical technologies. With the view to designing new functional materials, the physiological activity and cellular response of entrapped cells within different silica-based matrices have been assessed. A fine-tuning of the surface chemistry of the matrix has been achieved by the in situ copolymerization of an aqueous silica precursor and a biocompatible trifunctional silane bearing covalently bound neutral sugars. This method allows a facile control of chemical and physical interactions between the entrapped plant cells and the scaffold. The results show that the cell-matrix interaction has to be carefully controlled in order to avoid the mineralization of the cell wall which typically reduces the bioavailability of nutrients. Under appropriate conditions, the introduction of a trifunctional silane (ca. 10%) during the preparation of hybrid gels has shown to prolong the biological activity as well as the cellular viability of plant cells. The relations of cell behavior with some other key factors such as the porosity and the contraction of the matrix are also discussed.
将活体植物细胞封装到材料中可能为开发新的绿色生化技术提供了可能性。为了设计新的功能材料，评估了不同基于硅材料的基质中包封细胞的生理活性和细胞反应。通过原位共聚水相硅前体和带有共价结合的中性糖的生物相容性三官能硅烷，实现了基质表面化学的精细调整。该方法可以方便地控制包封植物细胞和支架之间的化学和物理相互作用。结果表明，为了避免细胞壁的矿化（通常会降低营养物质的生物利用度），必须仔细控制细胞-基质相互作用。在适当的条件下，在制备杂化凝胶的过程中引入三官能硅烷（约 10%）已显示出可延长植物细胞的生物活性和细胞活力。还讨论了细胞行为与其他一些关键因素（如多孔性和基质收缩）的关系。
