Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
FEMS Microbiol Lett. 2010 Mar;304(2):123-30. doi: 10.1111/j.1574-6968.2010.01897.x. Epub 2010 Jan 11.
Pseudomonas aeruginosa secretes membrane vesicles (MVs) that deliver several virulence factors as a cargo. We found that indole and its derivative compounds, including 4-hydroxyindole, 5-hydroxyindole, 6-hydroxyindole and isatin, repress MV production significantly. These compounds also repressed the synthesis of Pseudomonas quinolone signal (PQS), which is one of the quorum-sensing signals that upregulate virulence gene expression and positively control MV production. Moreover, we showed that other bicyclic compounds, including 1-naphthol, 2-naphthol, 2,3-dihydroxynaphthalene, 1-aminonaphthalene and 8-quinolinol, significantly repress MV production and PQS synthesis. In conclusion, we provide new information about the chemical structures that inhibit P. aeruginosa virulence.
铜绿假单胞菌分泌膜泡（MVs），将多种毒力因子作为货物运输。我们发现吲哚及其衍生物化合物，包括 4-羟基吲哚、5-羟基吲哚、6-羟基吲哚和靛蓝，能显著抑制 MV 的产生。这些化合物还抑制了铜绿假单胞菌群体感应信号（PQS）的合成，PQS 是上调毒力基因表达并正向调控 MV 产生的群体感应信号之一。此外，我们还表明，包括 1-萘酚、2-萘酚、2,3-二羟基萘、1-氨基萘和 8-羟基喹啉在内的其他双环化合物，也能显著抑制 MV 的产生和 PQS 的合成。总之，我们提供了关于抑制铜绿假单胞菌毒力的化学结构的新信息。
