Wu Y E, Zhang S W, Peng W G, Li K S, Li K, Jiang J K, Lin J H, Cai Y M
Department of Laboratory Medicine, First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China.
J Int Med Res. 2009 Nov-Dec;37(6):1742-9. doi: 10.1177/147323000903700610.
The aim of this study was to determine the percentage of lymphocyte subsets in peripheral blood in patients with active tuberculosis. A total of 21 patients with active tuberculosis and 15 healthy volunteers were included in the study. T-lymphocyte subsets, B-lymphocytes (CD19(+) cells), natural killer (NK) cells and cells positive for costimulatory molecules CD28 and CD152 were evaluated using flow cytometry. Patients with tuberculosis had a significantly decreased percentage of CD3(+) and CD3(+)CD4(+) cells, and a significantly decreased ratio of CD3(+)CD4(+) to CD3(+)CD8(+) cells compared with healthy controls. In contrast, the percentage of B-cells (CD19(+) cells), CD3(+)CD8(+) cells, CD28(+) cells, CD152(+) cells, and subpopulations of CD4(+)CD152(+), CD8(+)CD152(+) and CD8(+)CD28(+) T-cells were all significantly increased compared with healthy controls. There were no statistically significant differences in the percentages of NK cells or CD4(+)CD28(+) cells between patients and controls. These results indicate that patients with active tuberculosis have altered lymphocyte homeostasis.
本研究的目的是确定活动性肺结核患者外周血中淋巴细胞亚群的百分比。共有21例活动性肺结核患者和15名健康志愿者纳入本研究。使用流式细胞术评估T淋巴细胞亚群、B淋巴细胞（CD19(+)细胞）、自然杀伤（NK）细胞以及共刺激分子CD28和CD152阳性的细胞。与健康对照相比，肺结核患者CD3(+)和CD3(+)CD4(+)细胞的百分比显著降低，CD3(+)CD4(+)与CD3(+)CD8(+)细胞的比值也显著降低。相反，与健康对照相比，B细胞（CD19(+)细胞）、CD3(+)CD8(+)细胞、CD28(+)细胞、CD152(+)细胞以及CD4(+)CD152(+)、CD8(+)CD152(+)和CD8(+)CD28(+) T细胞亚群的百分比均显著增加。患者与对照之间NK细胞或CD4(+)CD28(+)细胞的百分比无统计学显著差异。这些结果表明，活动性肺结核患者的淋巴细胞稳态发生了改变。
