Department of Dermatology, Venereology and Allergology, Wrocław Medical University, Chalubinskiego 1, 50-368, Wroclaw, Poland.
Department of Clinical Immunology and Immunotherapy, Medical University of Lublin, Chodzki 4a Street, 20-093, Lublin, Poland.
Microb Pathog. 2019 Jan;126:85-91. doi: 10.1016/j.micpath.2018.10.019. Epub 2018 Oct 19.
Programmed cell death receptor 1 (PD-1) is one of the major immune checkpoints. Due to the lack of reports on PD-1- and PD-L1-positive lymphocyte proportions in patients with recurrent furunculosis, we aimed to evaluate percentages of those cells in the peripheral blood and to assess their correlations with other lymphocyte subsets, and the level of cell activation measured by the expression of CD25 and CD69 molecules on T lymphocytes. We recruited 30 patients with recurrent furunculosis and 15 controls. The amount of 5 mL of peripheral blood was collected for laboratory tests. Patients with chronic furunculosis presented with the similar number of lymphocytes, CD4 T lymphocytes, CD8CD3 T suppressor lymphocytes, and CD19 B lymphocytes to controls, but significant differences were found between subpopulation of those cells. Furunculosis patients had the significantly elevated percentage of lymphocytes with PD-1 and PD-L1 on their surface. Early onset of furunculosis was correlated with a higher percentage of CD19 + PD1 B lymphocytes. Greater number of skin lesions correlated with a decrease in the CD4PDL1+ cells, which subsequently was associated with an increase in the percentage of Treg cells, NKT cells, CD8CD3 lymphocytes and B lymphocytes. Changes in the proportion of immune cells may lead to reduced inflammatory reactions in patients with recurrent furunculosis. In the light of mechanisms of S. aureus invasion, the degree of immune impairments in the scope of adaptive immunity seems to play a significant role in the course of furunculosis. PD-1 and PD-L1 molecules change the host response and affect the ongoing inflammatory process.
程序性细胞死亡受体 1（PD-1）是主要的免疫检查点之一。由于缺乏复发性疖患者 PD-1 和 PD-L1 阳性淋巴细胞比例的报道，我们旨在评估外周血中这些细胞的百分比，并评估它们与其他淋巴细胞亚群的相关性，以及 T 淋巴细胞上 CD25 和 CD69 分子表达所测量的细胞激活水平。我们招募了 30 名复发性疖患者和 15 名对照。采集 5 mL 外周血进行实验室检查。慢性疖患者的淋巴细胞、CD4 T 淋巴细胞、CD8CD3 T 抑制性淋巴细胞和 CD19 B 淋巴细胞数量与对照组相似，但这些细胞亚群之间存在显著差异。疖患者表面有 PD-1 和 PD-L1 的淋巴细胞比例显著升高。疖的早期发病与 CD19+PD1 B 淋巴细胞的百分比升高有关。皮损数量较多与 CD4PDL1+细胞减少相关，随后与 Treg 细胞、NKT 细胞、CD8CD3 淋巴细胞和 B 淋巴细胞百分比增加相关。免疫细胞比例的变化可能导致复发性疖患者炎症反应减轻。鉴于金黄色葡萄球菌入侵的机制，适应性免疫范围内免疫损伤的程度似乎在疖的病程中起着重要作用。PD-1 和 PD-L1 分子改变宿主反应并影响正在进行的炎症过程。
