选择用于小鼠RNA干扰的双链RNA序列。
Choosing the sequence of dsRNA for RNAi in mouse.
作者信息
Svoboda Petr
机构信息
Institute of Molecular Genetics, Academy of Sciences of Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic.
出版信息
Cold Spring Harb Protoc. 2009 Jan;2009(1):pdb.ip63. doi: 10.1101/pdb.ip63.
DOI:10.1101/pdb.ip63
PMID:20147010
Abstract
RNA interference (RNAi) is a suitable method for sequence-specific post-transcriptional gene silencing for a number of model systems. Here, we describe selection of the target sequence for efficient RNAi knockdown in mouse.
摘要
RNA干扰（RNAi）对于许多模型系统而言，是一种适用于序列特异性转录后基因沉默的方法。在此，我们描述了在小鼠中进行有效RNAi敲低的靶序列选择。
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