Brent Colin, Dolezal Adam
USDA Arid-Land Agricultural Research Center, Maricopa, AZ 85238, USA.
Cold Spring Harb Protoc. 2009 Jul;2009(7):pdb.prot5246. doi: 10.1101/pdb.prot5246.
Juvenile hormone (JH) is an important insect hormone known to have many effects on development, reproduction, and behavior in both solitary and social insects. A number of questions using ants as a model involve JH. This procedure allows for quantification of circulating levels of JH III, which can be an important factor in many questions relating to insect research. The JH III is extracted from a subject, purified, and converted to a d(3)-methoxyhydrin derivative that can be quantified by gas chromatography-mass spectrometry (GC-MS). The major advantages of this protocol are its high resolution, and its ability to quantify significant differences between relatively small quantities of the hormone. Its major limitations are the time necessary to process samples, its relatively high cost, and maintaining the sensitivity of the equipment.
保幼激素（JH）是一种重要的昆虫激素，已知其对独居昆虫和群居昆虫的发育、繁殖及行为均有诸多影响。以蚂蚁为模型的许多问题都涉及保幼激素。此程序可对保幼激素III的循环水平进行定量，而保幼激素III可能是许多与昆虫研究相关问题的重要因素。保幼激素III从实验对象中提取、纯化，然后转化为可通过气相色谱 - 质谱联用仪（GC - MS）进行定量的d(3)-甲氧基醇衍生物。该方案的主要优点是分辨率高，且能够对相对少量激素之间的显著差异进行定量。其主要局限性在于处理样品所需的时间、相对较高的成本以及维持设备的灵敏度。
