靶向蛋白降解的化学诱导剂。
Chemical inducers of targeted protein degradation.
机构信息
Department of Chemistry, Yale University, New Haven, Connecticut 06520-8103, USA.
出版信息
J Biol Chem. 2010 Apr 9;285(15):11057-60. doi: 10.1074/jbc.R109.078105. Epub 2010 Feb 10.
Abstract
The functions of many cellular proteins have been elucidated by selective gene inactivation and subsequent phenotypic analysis. For example, genetic mutations, gene knock-out generation, and the use of RNA interference to target mRNA for degradation can all result in decreased production of a specific protein, yielding informative cellular phenotypes. However, these techniques each have certain inherent limitations. This minireview focuses on the recent development of new approaches to study protein function at the post-translational level, namely chemical induction of targeted protein degradation.
摘要
许多细胞蛋白的功能已通过选择性基因失活和随后的表型分析阐明。例如，遗传突变、基因敲除的产生，以及使用 RNA 干扰来靶向 mRNA 进行降解，都可能导致特定蛋白质的产量降低，从而产生有意义的细胞表型。然而，这些技术各自都存在一定的固有局限性。这篇综述性短文重点介绍了在翻译后水平研究蛋白质功能的新方法的最新进展，即靶向蛋白降解的化学诱导。
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2
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Bioorg Med Chem Lett. 2008 Nov 15;18(22):5941-4. doi: 10.1016/j.bmcl.2008.09.043. Epub 2008 Sep 12.
3
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Bioorg Med Chem Lett. 2008 Nov 15;18(22):5904-8. doi: 10.1016/j.bmcl.2008.07.114. Epub 2008 Jul 31.
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5
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Chembiochem. 2007 Nov 23;8(17):2058-62. doi: 10.1002/cbic.200700438.
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