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限制性内切酶标记基因组扫描方法在F(1)杂种非孟德尔遗传监测中的应用。

The application of restriction landmark genome scanning method for surveillance of non-mendelian inheritance in f(1) hybrids.

作者信息

Takamiya Tomoko, Hosobuchi Saeko, Noguchi Tomotsugu, Paterson Andrew H, Iijima Hiroshi, Murakami Yasufumi, Okuizumi Hisato

机构信息

Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences (NIAS), Tsukuba, Ibaraki 305-8602, Japan.

出版信息

Comp Funct Genomics. 2009;2009:245927. doi: 10.1155/2009/245927. Epub 2010 Jan 27.

DOI:10.1155/2009/245927
PMID:20148066
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2817499/
Abstract

We analyzed inheritance of DNA methylation in reciprocal F(1) hybrids (subsp. japonica cv. Nipponbare x subsp. indica cv. Kasalath) of rice (Oryza sativa L.) using restriction landmark genome scanning (RLGS), and detected differing RLGS spots between the parents and reciprocal F(1) hybrids. MspI/HpaII restriction sites in the DNA from these different spots were suspected to be heterozygously methylated in the Nipponbare parent. These spots segregated in F(1) plants, but did not segregate in selfed progeny of Nipponbare, showing non-Mendelian inheritance of the methylation status. As a result of RT-PCR and sequencing, a specific allele of the gene nearest to the methylated sites was expressed in reciprocal F(1) plants, showing evidence of biased allelic expression. These results show the applicability of RLGS for scanning of non-Mendelian inheritance of DNA methylation and biased allelic expression.

摘要

我们利用限制性内切酶标记基因组扫描（RLGS）分析了水稻（Oryza sativa L.）正反交F(1)杂种（粳稻品种日本晴×籼稻品种Kasalath）中DNA甲基化的遗传情况，并在亲本和正反交F(1)杂种之间检测到不同的RLGS斑点。怀疑来自这些不同斑点的DNA中的MspI/HpaII限制性位点在日本晴亲本中是杂合甲基化的。这些斑点在F(1)植株中分离，但在日本晴的自交后代中不分离，表明甲基化状态呈非孟德尔遗传。通过RT-PCR和测序，在正反交F(1)植株中表达了最接近甲基化位点的基因的一个特定等位基因，显示出等位基因表达偏向的证据。这些结果表明RLGS可用于扫描DNA甲基化的非孟德尔遗传和等位基因表达偏向。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cb5/2817499/c50f0510fd84/CFG2009-245927.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cb5/2817499/dd022efc972a/CFG2009-245927.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cb5/2817499/16a2d47039b3/CFG2009-245927.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cb5/2817499/f44eea7c693e/CFG2009-245927.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cb5/2817499/c50f0510fd84/CFG2009-245927.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cb5/2817499/dd022efc972a/CFG2009-245927.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cb5/2817499/16a2d47039b3/CFG2009-245927.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cb5/2817499/f44eea7c693e/CFG2009-245927.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cb5/2817499/c50f0510fd84/CFG2009-245927.004.jpg

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