Takamiya Tomoko, Hosobuchi Saeko, Noguchi Tomotsugu, Asai Kenji, Nakamura Eiji, Habu Yoshiki, Paterson Andrew H, Iijima Hiroshi, Murakami Yasufumi, Okuizumi Hisato
Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences (NIAS), Tsukuba, Ibaraki, Japan.
Electrophoresis. 2008 Oct;29(19):4088-95. doi: 10.1002/elps.200700784.
We analyzed the inheritance of DNA methylation in the first filial generation(F1) hybrid of Oryza sativa L. ("Nipponbare"x"Kasalath") by restriction landmark genome scanning (RLGS). Most parental RLGS spots were found in the F1, but eight spots (4%) showed abnormal inheritance: seven of the eight spots were missing in the F1, and one was newly detected in the F1. Here we show demethylation at restriction enzyme sites in the F1. We also found a candidate site of stable heterozygous methylation in the genome. These results show the applicability of the RLGS method for analysis of the inheritance and alteration of methylation in F1 hybrid plants.
我们通过限制性内切酶标记基因组扫描（RLGS）分析了水稻（“日本晴”ד卡萨拉斯”）第一代杂交种（F1）中DNA甲基化的遗传情况。在F1中发现了大多数亲本RLGS位点，但有8个位点（4%）表现出异常遗传：8个位点中的7个在F1中缺失，1个在F1中被新检测到。在此我们展示了F1中限制性酶切位点处的去甲基化现象。我们还在基因组中发现了一个稳定杂合甲基化的候选位点。这些结果表明RLGS方法适用于分析F1杂交植物中甲基化的遗传和变化情况。
