Page T, Moore G M, Will J, Halloran G M
Agroforestry and Novel Crops Unit, School of Marine and Tropical Biology, James Cook University, PO Box 6811, Cairns, QLD, 4870, Australia.
Sex Plant Reprod. 2010 Sep;23(3):239-53. doi: 10.1007/s00497-010-0133-0. Epub 2010 Feb 11.
To examine breeding system characteristics of the endemic Australian prostrate shrub Kunzea pomifera, artificial hybridisations were undertaken using thirteen different genotypes of K. pomifera, to elucidate: (1) self-incompatibility, (2) intraspecific cross-compatibility in the species and (3) interspecific cross-compatibility with each of K. ambigua and K. ericoides. K. pomifera exhibited very low self-compatibility, with the barrier to self-fertilisation being prevention of pollen-tube growth in the style or ovary. Following intraspecific pollination amongst a number of different genotypes of K. pomifera, 38.4% of pollinated flowers developed fruit; arrest of compatible pollen-tubes in the style, preventing fertilisation, contributes to the low fruit set in this species. Interspecific compatibility was examined between K. pomifera (pistillate parent) and K. ambigua (staminate parent) where seed set per pollinated flower (4.47) was not significantly different from intraspecific crosses (4.66). In crosses between K. pomifera (pistillate parent) and K. ericoides as staminate plant, 0.037% of pollinated flowers produced fruit, with 0.0075 seeds per pollinated flower. Reproductive barriers between these two species were evident in the style of K. pomifera, where the growing tips of the K. ericoides pollen-tubes swelled and ceased to grow.
为研究澳大利亚特有的匍匐灌木多籽昆泽木的繁育系统特征，利用13种不同基因型的多籽昆泽木进行了人工杂交，以阐明：（1）自交不亲和性；（2）该物种的种内杂交亲和性；（3）与安比昆泽木和拟南芥昆泽木的种间杂交亲和性。多籽昆泽木表现出极低的自交亲和性，自花受精的障碍在于阻止花粉管在花柱或子房内生长。在多种不同基因型的多籽昆泽木进行种内授粉后，38.4%的授粉花发育成果实；花柱中亲和花粉管的停滞阻止了受精，导致该物种的坐果率较低。研究了多籽昆泽木（雌蕊亲本）与安比昆泽木（雄蕊亲本）之间的种间亲和性，每朵授粉花的结实率（4.47）与种内杂交（4.66）无显著差异。在多籽昆泽木（雌蕊亲本）与作为雄蕊植物的拟南芥昆泽木的杂交中，0.037%的授粉花结果，每朵授粉花有0.0075粒种子。这两个物种之间的生殖障碍在多籽昆泽木的花柱中很明显，拟南芥昆泽木花粉管的生长尖端肿胀并停止生长。
