Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge, Massachusetts 02139, USA.
Biotechnol Bioeng. 2010 Jun 1;106(2):319-25. doi: 10.1002/bit.22688.
The production of heterologous proteins by secretion from cellular hosts is an important determinant for the cost of biotherapeutics. A single-cell analytical method called microengraving was used to examine the heterogeneity in secretion by the methylotrophic yeast Pichia pastoris. We show that constitutive secretion of a human Fc fragment by P. pastoris is not cell-cycle dependent, but rather fluctuates between states of high and low productivity in a stochastic manner.
细胞宿主通过分泌生产异源蛋白是生物疗法成本的一个重要决定因素。一种称为微刻蚀的单细胞分析方法被用于研究甲基营养酵母巴斯德毕赤酵母（Pichia pastoris）分泌的异质性。我们表明，巴斯德毕赤酵母组成型分泌人 Fc 片段与细胞周期无关，而是以随机的方式在高生产力和低生产力状态之间波动。
