CRP-Gabriel Lippmann, Department Environment and Agro-biotechnologies, Belvaux, GD, Luxembourg.
Proteomics. 2010 Feb;10(3):349-68. doi: 10.1002/pmic.200900484.
The comprehension of metal homeostasis in plants requires the identification of molecular markers linked to stress tolerance. Proteomic changes in leaves and cambial zone of Populus tremula x P. alba (717-1B4 genotype) were analyzed after 61 days of exposure to cadmium (Cd) 360 mg/kg soil dry weight in pot-soil cultures. The treatment led to an acute Cd stress with a reduction of growth and photosynthesis. Cd stress induced changes in the display of 120 spots for leaf tissue and 153 spots for the cambial zone. It involved a reduced photosynthesis, resulting in a profound reorganisation of carbon and carbohydrate metabolisms in both tissues. Cambial cells underwent stress from the Cd actually present inside the tissue but also a deprivation of photosynthates caused by leaf stress. An important tissue specificity of the response was observed, according to the differences in cell structures and functions.
植物金属内稳性的理解需要鉴定与胁迫耐受性相关的分子标记。在盆栽土壤培养中，将 717-1B4 基因型的欧洲山杨×银白杨（Populus tremula x P. alba）叶片和形成层区暴露于土壤干重 360mg/kg 的镉（Cd）61 天后，分析了叶片和形成层区的蛋白质组变化。该处理导致急性 Cd 胁迫，生长和光合作用减少。Cd 胁迫诱导叶片组织显示 120 个斑点和形成层区显示 153 个斑点的变化。它涉及光合作用的减少，导致两个组织中碳和碳水化合物代谢的深刻重组。形成层细胞受到组织内存在的 Cd 的胁迫，还受到叶片胁迫引起的光合产物剥夺的胁迫。根据细胞结构和功能的差异，观察到了重要的组织特异性反应。
