Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.
J Agric Food Chem. 2010 Mar 10;58(5):2801-6. doi: 10.1021/jf903897k.
A magnetic particle-based enzyme-linked immunosorbent assay (mp-ELISA) to detect multiresidue organophosphorus pesticides (OPs) was developed and evaluated in parallel with a conventional competitive indirect ELISA utilizing the same pair of antibodies. The antibody was immobilized by chemical coupling on the ferroferric oxide nanoparticles coated with 3-aminopropyl triethoxysilane (APTES). Comparative studies demonstrated that mp-ELISA exhibited both significantly improved sensitivity and better class specificity than conventional ELISA, although the reproducibility and repeatability of the two assays were equivalent. As compared with data from the conventional ELISA, the averages of the midpoint inhibition (IC(50)) decreasing rate of mp-ELISA were 81.1 and 62.1% for the magnetic first antibody (MFA) and magnetic second antibody (MSA), respectively. The class specificity of MFA was broader than that of MSA. The results demonstrated the high potential of mp-ELISA based on the MFA as a tool to improve sensitivity and broad specificity in multiresidue immunoassay.
一种基于磁性粒子的酶联免疫吸附测定法（mp-ELISA）被开发出来，并与利用相同抗体的传统竞争性间接 ELISA 同时进行评估，用于检测多残留有机磷农药（OPs）。抗体通过化学偶联固定在涂有 3-氨丙基三乙氧基硅烷（APTES）的四氧化三铁纳米粒子上。对比研究表明，mp-ELISA 与传统 ELISA 相比，不仅具有显著提高的灵敏度，而且具有更好的类特异性，尽管两种检测方法的重现性和重复性相当。与传统 ELISA 的数据相比，mp-ELISA 的磁抗体（MFA）和磁二抗（MSA）的半数抑制（IC50）降低率平均值分别为 81.1%和 62.1%。MFA 的类特异性比 MSA 更宽。结果表明，基于 MFA 的 mp-ELISA 具有作为提高多残留免疫分析灵敏度和广谱特异性的工具的高潜力。
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