Tang Jianshe, Zhang Min, Cheng Guohua, Li Ang, Lu Yitong
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
J Environ Sci Health B. 2008 Nov;43(8):707-12. doi: 10.1080/03601230802388827.
Diethyl (carboxymethyl) phosphonate (DECP) was used as the hapten to develop an indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) for detecting organophosphorus pesticides (OPs). Conjugator of DECP with bovin serum albumin (BSA) was used as the immunogen for producing the polyclonal antibodies (PcAbs). Three antisera were obtained after the immune procedure. Characterization studies of the PcAbs indicated that the titer of antiserum-1 was highest in 3 antisera, and antiserum-1 had high affinity and specificity to the parathion, dichlorvos and pirimiphos. The IC-ELISA showed an IC50 of 0.428 micro g/mL with a detection limit of 0.0125 micro g/mL to parathion. The assay also indicated that the IC50 values of pirimiphos and dichlorvos were 0.331 micro g/mL and 1.25 micro g/mL respectively, and the detection limits of pirimiphos and dichlorvos were 0.0116 micro g/mL and 0.048 micro g/mL respectively. Recoveries of parathion, pirimiphos and dichlorvos spiked into water samples ranged from 90% to 160%. The results indicated that the ELISA could be a convenient and supplemental analytical tool for monitoring OPs residues in environmental water samples.
以二乙基（羧甲基）膦酸酯（DECP）作为半抗原，建立了一种用于检测有机磷农药（OPs）的间接竞争酶联免疫吸附测定法（IC-ELISA）。将DECP与牛血清白蛋白（BSA）的偶联物用作免疫原，以制备多克隆抗体（PcAbs）。免疫程序后获得了三种抗血清。对PcAbs的表征研究表明，抗血清1在三种抗血清中的效价最高，并且抗血清1对对硫磷、敌敌畏和嘧啶磷具有高亲和力和特异性。IC-ELISA对硫磷的IC50为0.428μg/mL，检测限为0.0125μg/mL。该测定还表明，嘧啶磷和敌敌畏的IC50值分别为0.331μg/mL和1.25μg/mL，嘧啶磷和敌敌畏的检测限分别为0.0116μg/mL和0.048μg/mL。添加到水样中的对硫磷、嘧啶磷和敌敌畏的回收率在90%至160%之间。结果表明，该ELISA可作为监测环境水样中OPs残留的一种方便且补充性的分析工具。
