Anal Chem. 2010 Mar 1;82(5):1580-3. doi: 10.1021/ac902223y.
Capillary isoelectric focusing (CIEF) was first performed in an open tubular mode. It depended on an immobilized pH gradients from the polymerization of several solutions, which contained acrylamide, N,N'-methylenebisacrylamide, allylamine, and acrylic acid. Different ratios of the basic and acidic monomers lead to solutions of various pHs. When injected into a capillary in the sequence of their pHs, the solutions polymerized and resulted into polymers with a series of isoelectric points (pIs). These polymers formed a covalently attached hydrophilic layer in the capillary, acting as both column bonding and carrier ampholytes. When five polymers were orderly lined up in a capillary, an immobilized pH gradient was established. This open tubular column was subjected to CIEF, showing excellent performance properties for protein focusing.
毛细管等电聚焦（CIEF）最初以开放式管状模式进行。它依赖于几种溶液聚合形成的固定 pH 梯度，这些溶液包含丙烯酰胺、N,N'-亚甲基双丙烯酰胺、烯丙胺和丙烯酸。不同比例的碱性和酸性单体导致溶液具有不同的 pH 值。当按 pH 值顺序注入毛细管时，溶液聚合形成一系列等电点（pI）的聚合物。这些聚合物在毛细管中形成共价键合的亲水层，既作为柱键合，又作为载体两性电解质。当将 5 种聚合物有序排列在毛细管中时，就建立了固定 pH 梯度。这种开放式管状柱用于 CIEF，显示出优异的蛋白质聚焦性能。
