Bioquímica Investigación, Fundación Jiménez Díaz, 28040 Madrid, Spain.
Aging Male. 2010 Mar;13(1):59-73. doi: 10.3109/13685530903536650.
Rat parathyroid hormone (PTH) 1-34 (4 microg/kg/day) was applied for 2.5 months to 9 month-old rats immediately after ovariectomy or orchidectomy or to 15 month-old rats with low bone mass which had been castrated 6 months before in order to know the effects on serum biochemistry parameters, lumbar and femoral bone mineral density, histology, cancellous and cortical bone histomorphometry, mineralisation content profile in cortical bone by backscattered-electron microscopy, and femoral torsion biomechanical testing. In ovariectomised rats, preventive PTH treatment avoided cancellous bone loss in tibial metaphysis and partially in lumbar vertebra, while in cortical bone, PTH increased endosteal resorption and periosteal formation. In intervention study, PTH did not restore cancellous bone but a strong endosteal and periosteal new bone formation was detected. In orchidectomised rats, PTH, in preventive study, avoided cancellous bone loss in metaphysis and lumbar vertebra, and a mild new bone formation in cortical bone was found. In intervention study, PTH maintained baseline cancellous bone mass, but in cortical bone a strong endosteal and periosteal new bone formation was detected. The PTH-induced new bone formation was confirmed by histology and by mineral content profiles. After castration, biomechanical properties were affected in females but not in male rats and PTH reverted this effect.
大鼠甲状旁腺激素（PTH）1-34（4μg/kg/天）在卵巢切除术或去势后立即应用于 9 月龄大鼠，或在 6 个月前因低骨量而被去势的 15 月龄大鼠中应用 2.5 个月，以了解其对血清生化参数、腰椎和股骨骨密度、组织学、松质骨和皮质骨组织形态计量学、皮质骨矿化含量分布的影响通过背散射电子显微镜，以及股骨扭转生物力学测试。在卵巢切除大鼠中，预防性 PTH 治疗可避免胫骨干骺端和部分腰椎的松质骨丢失，而在皮质骨中，PTH 增加了骨内膜吸收和骨膜形成。在干预研究中，PTH 并未恢复松质骨，但检测到强烈的骨内膜和骨膜新骨形成。在去势大鼠中，预防性研究中 PTH 可避免干骺端和腰椎的松质骨丢失，并在皮质骨中发现轻度新骨形成。在干预研究中，PTH 维持了皮质骨的基线骨量，但在皮质骨中检测到强烈的骨内膜和骨膜新骨形成。PTH 诱导的新骨形成通过组织学和矿化含量分布得到证实。去势后，女性的生物力学性能受到影响，但雄性大鼠不受影响，而 PTH 则逆转了这种影响。
