Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology (CDB), Kobe, Hyogo, Japan.
Dev Growth Differ. 2010 Apr;52(3):275-83. doi: 10.1111/j.1440-169X.2009.01166.x. Epub 2010 Feb 10.
Mouse embryonic stem (mES) cells are pluripotent stem cells derived from pre-implantation embryos. They are regarded as an essential tool for studying mouse development, as they provide a means for generating knock-out mouse lines. This, however, is not the sole utility of the mES cell system. They undergo differentiation in culture, mimicking the morphological differentiation of peri-implantation embryos from epiblast to egg-cylinder stage. Moreover, they retain the capacity to respond to triggers of differentiation toward trophectoderm and primitive endoderm by forced activation. For these reasons, mES cells can be regarded as a useful tool for analyzing molecular mechanisms underlying early mouse development.
鼠胚胎干细胞（mES 细胞）是一种多能干细胞，来源于着床前胚胎。它们被认为是研究鼠类发育的重要工具，因为它们提供了一种生成基因敲除鼠系的方法。然而，mES 细胞系统的应用并不仅限于此。它们在培养过程中经历分化，模拟着床前胚胎从外胚层到囊胚阶段的形态分化。此外，它们通过强制激活保留了对滋养层和原始内胚层分化触发的反应能力。由于这些原因，mES 细胞可被视为分析早期鼠类发育分子机制的有用工具。
