Sox7对于小鼠胚胎干细胞向内胚层的原始分化并非必需。
Sox7 is dispensable for primitive endoderm differentiation from mouse ES cells.
作者信息
Kinoshita Masaki, Shimosato Daisuke, Yamane Mariko, Niwa Hitoshi
机构信息
Laboratory for Pluripotent cell studies, RIKEN, Centre for Developmental Biology, 2-2-3, Minatojima-Minamimachi, Chuo-ku, Kobe, Hyogo, 650-0047, Japan.
Laboratory for Development and Regenerative Medicine, Kobe University Graduate School of Medicine, 7-5-1 Kusunokicho, Chuo-ku, Kobe, Hyogo, 650-0017, Japan.
出版信息
BMC Dev Biol. 2015 Oct 16;15:37. doi: 10.1186/s12861-015-0079-4.
BACKGROUND
Primitive endoderm is a cell lineage segregated from the epiblast in the blastocyst and gives rise to parietal and visceral endoderm. Sox7 is a member of the SoxF gene family that is specifically expressed in primitive endoderm in the late blastocyst, although its function in this cell lineage remains unclear.
RESULTS
Here we characterize the function of Sox7 in primitive endoderm differentiation using mouse embryonic stem (ES) cells as a model system. We show that ectopic expression of Sox7 in ES cells has a marginal effect on triggering differentiation into primitive endoderm-like cells. We also show that targeted disruption of Sox7 in ES cells does not affect differentiation into primitive endoderm cells in embryoid body formation as well as by forced expression of Gata6.
CONCLUSIONS
These data indicate that Sox7 function is supplementary and not essential for this differentiation from ES cells.
背景
原始内胚层是在囊胚中从外胚层分离出来的细胞谱系，可分化为壁内胚层和脏内胚层。Sox7是SoxF基因家族的成员，在囊胚晚期的原始内胚层中特异性表达，但其在该细胞谱系中的功能尚不清楚。
结果
在此，我们以小鼠胚胎干细胞（ES细胞）为模型系统，对Sox7在原始内胚层分化中的功能进行了表征。我们发现，ES细胞中Sox7的异位表达对触发其分化为原始内胚层样细胞的影响很小。我们还表明，在ES细胞中靶向破坏Sox7，并不影响胚胎体形成过程中以及通过强制表达Gata6向原始内胚层细胞的分化。
结论
这些数据表明，Sox7的功能对于ES细胞的这种分化是辅助性的，而非必需的。
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