Department of Clinical Pathology, Showa University Fujigaoka Hospital, Aoba-ku, Yokohama, Japan.
Infection Control Team, Showa University Fujigaoka Hospital, Aoba-ku, Yokohama, Japan.
J Appl Microbiol. 2010 Aug;109(2):558-566. doi: 10.1111/j.1365-2672.2010.04680.x. Epub 2010 Jan 22.
To better understand nontuberculous mycobacteria (NTM) contamination in a hospital setting, six freshwater fish gut homogenates and water in an aquarium fish tank placed on the reception counter of a nursing station were cultured for mycobacteria.
By direct sequencing of 16s rRNA, rpoB and hsp65, scotochromogenic and nonchromogenic Mycobacterium szulgai isolates containing hsp65 type II (GenBank accession nos. FJ384762 and FJ384764, respectively), Mycobacterium gordonae isolates containing rpoB clusters B and E (GenBank accession no. FJ384766), and Mycobacterium kansasii isolates containing hsp65 type VI were collected from the gut homogenates and water from the fish tank. However, no isolates were obtained from the tap water used to refill the fish tank. A randomly amplified polymorphic DNA (RAPD) analysis using a 10-mer primer (5'-TGGTCGCGGC) showed that some NTM from the fish tank water were identical to those obtained from the gut homogenates.
Fish and water in the tank were contaminated by the novel NTM.
These findings could help to elucidate infection routes and contamination sources of novel NTM from water sources.
为了更好地了解医院环境中非结核分枝杆菌（NTM）的污染情况，对放在护士站接待台上鱼缸中的 6 种淡水鱼的肠道匀浆和水进行了分枝杆菌培养。
通过对 16s rRNA、rpoB 和 hsp65 的直接测序，从肠道匀浆和水箱水中分离出含有 hsp65 Ⅱ型（GenBank 登录号分别为 FJ384762 和 FJ384764）的 scotochromogenic 和 nonchromogenic Mycobacterium szulgai 分离株、含有 rpoB 簇 B 和 E（GenBank 登录号为 FJ384766）的 Mycobacterium gordonae 分离株和含有 hsp65 Ⅵ型的 Mycobacterium kansasii 分离株。然而，从用于给鱼缸补水的自来水中未分离到任何分离株。使用 10 聚体引物（5'-TGGTCGCGGC）进行随机扩增多态性 DNA（RAPD）分析显示，来自水箱水的一些 NTM 与从肠道匀浆中获得的 NTM 相同。
鱼缸中的鱼和水受到了新型 NTM 的污染。
这些发现有助于阐明来自水源的新型 NTM 的感染途径和污染来源。
