人绒毛膜促性腺激素对雌性大鼠受刺激逼尿肌条体外收缩的影响。

Effect of human chorionic gonadotrophin on in vitro contractions of stimulated detrusor muscle strips of female rats.

作者信息

Rizk Diaa E E, Osman Nawal A, Shafiullah Mohammed M, Nagelkerke Nicolaas J D, Fahim Mohammed A

机构信息

Department of Obstetrics and Gynecology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al-Ain, United Arab Emirates.

出版信息

J Obstet Gynaecol Res. 2009 Oct;35(5):835-41. doi: 10.1111/j.1447-0756.2009.01052.x.

DOI:10.1111/j.1447-0756.2009.01052.x

PMID:20149029

Abstract

AIMS

We studied the effect of human chorionic gonadotrophin (hCG) on the in vitro detrusor muscle contractions in female rats.

METHODS

Two adjacent detrusor muscle strips from the bladder dome of 18 female Wistar rats (230-250 gm) were mounted in an organ bath for the recording of isometric tension. Carbachol (10(-9)-10(-3) M), alpha,beta methylene adenosine 5'-triphosphate (ATP) (10(-9)-10(-3) M) and potassium chloride (KCl) (10(-4)-10(-3) M) were applied (n = 6 x 3 groups). Concentration-response curves, before and after the addition of hCG (100 iu/mL) or oxybutynin (10(-5) M) to either muscle strip, were compared.

RESULTS

All curves were displaced to the right by hCG in a concentration-dependent manner with significant inhibition of contractions induced by carbachol (P < 0.001) and KCl (P = 0.016) but not those induced by alpha,beta-methylene ATP (P = 0.4). Estimated order of potency of inhibition was carbachol>KCl>alpha,beta-methylene ATP. The overall inhibitory effect of hCG was significantly less than oxybutynin (P < 0.001).

CONCLUSIONS

hCG significantly inhibited in vitro detrusor contractions induced by depolarization (KCl) and cholinergic (carbachol) but not purinergic (alpha,beta-methylene ATP) stimulation in a dose-dependent manner in female rats.

摘要

目的

我们研究了人绒毛膜促性腺激素（hCG）对雌性大鼠离体逼尿肌收缩的影响。

方法

从18只雌性Wistar大鼠（230 - 250克）膀胱顶部获取两条相邻的逼尿肌条带，安装在器官浴槽中记录等长张力。应用卡巴胆碱（10⁻⁹ - 10⁻³ M）、α,β - 亚甲基腺苷5'-三磷酸（ATP）（10⁻⁹ - 10⁻³ M）和氯化钾（KCl）（10⁻⁴ - 10⁻³ M）（n = 6×3组）。比较在任一肌条添加hCG（100 iu/mL）或奥昔布宁（10⁻⁵ M）前后的浓度 - 反应曲线。

结果

hCG使所有曲线以浓度依赖方式右移，对卡巴胆碱（P < 0.001）和KCl（P = 0.016）诱导的收缩有显著抑制作用，但对α,β - 亚甲基ATP诱导的收缩无抑制作用（P = 0.4）。估计抑制效力顺序为卡巴胆碱>KCl>α,β - 亚甲基ATP。hCG的总体抑制作用明显小于奥昔布宁（P < 0.001）。