School of Life Science and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
Bioresour Technol. 2010 Jun;101(11):4157-61. doi: 10.1016/j.biortech.2010.01.066. Epub 2010 Feb 9.
Saccharomyces cerevisiae was metabolically engineered to improve ethanol production from glycerol. High rates of glycerol utilization were achieved by simultaneous overexpression of glycerol dehydrogenase (Gcy) and dihydroxyacetone kinase (Dak), which are the enzymes responsible for the conversion of glycerol to glycolytic intermediate dihydroxyacetone phosphate. As a result, ethanol production in YPH499 (pGcyaDak) was about 2.4-fold higher than wild strain. We have also successfully expressed a glycerol uptake protein (Gup1). The overall ethanol production in strain YPH499 (pGcyaDak, pGupCas) was 3.4-fold more than in wild strain, with about 2.4gL(-1) ethanol produced. These experimental results confirmed our metabolic pathway strategies which improve the production of ethanol.
酿酒酵母经代谢工程改造以提高甘油生产乙醇的能力。通过甘油脱氢酶 (Gcy) 和二羟丙酮激酶 (Dak) 的同时过表达，实现了甘油的高利用率，这两种酶负责将甘油转化为糖酵解中间体二羟丙酮磷酸。结果，YPH499(pGcyaDak) 中的乙醇产量比野生型菌株高约 2.4 倍。我们还成功表达了甘油摄取蛋白 (Gup1)。在 YPH499 菌株 (pGcyaDak, pGupCas) 中的总乙醇产量比野生型菌株高出 3.4 倍，约产生 2.4gL(-1)的乙醇。这些实验结果证实了我们的代谢途径策略，可提高乙醇的产量。
