Jansen Mickel L A, Bracher Jasmine M, Papapetridis Ioannis, Verhoeven Maarten D, de Bruijn Hans, de Waal Paul P, van Maris Antonius J A, Klaassen Paul, Pronk Jack T
DSM Biotechnology Centre, Alexander Fleminglaan 1, 2613 AX Delft, The Netherlands.
Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The Netherlands.
FEMS Yeast Res. 2017 Aug 1;17(5). doi: 10.1093/femsyr/fox044.
The recent start-up of several full-scale 'second generation' ethanol plants marks a major milestone in the development of Saccharomyces cerevisiae strains for fermentation of lignocellulosic hydrolysates of agricultural residues and energy crops. After a discussion of the challenges that these novel industrial contexts impose on yeast strains, this minireview describes key metabolic engineering strategies that have been developed to address these challenges. Additionally, it outlines how proof-of-concept studies, often developed in academic settings, can be used for the development of robust strain platforms that meet the requirements for industrial application. Fermentation performance of current engineered industrial S. cerevisiae strains is no longer a bottleneck in efforts to achieve the projected outputs of the first large-scale second-generation ethanol plants. Academic and industrial yeast research will continue to strengthen the economic value position of second-generation ethanol production by further improving fermentation kinetics, product yield and cellular robustness under process conditions.
最近几家全规模“第二代”乙醇工厂的投产标志着用于发酵农业残留物和能源作物木质纤维素水解产物的酿酒酵母菌株开发中的一个重要里程碑。在讨论了这些新型工业环境对酵母菌株带来的挑战之后，本综述描述了为应对这些挑战而开发的关键代谢工程策略。此外，它概述了通常在学术环境中开展的概念验证研究如何能够用于开发满足工业应用要求的强大菌株平台。目前工程化的工业酿酒酵母菌株的发酵性能已不再是实现首批大型第二代乙醇工厂预期产量的瓶颈。学术和工业酵母研究将通过进一步改善发酵动力学、产物产量以及工艺条件下的细胞稳健性，继续加强第二代乙醇生产的经济价值地位。
