Covance Laboratories Limited, Otley Road, Harrogate, United Kingdom.
Mutat Res. 2010 Oct 29;702(2):139-47. doi: 10.1016/j.mrgentox.2010.02.001. Epub 2010 Feb 10.
This paper summarises the data for 14 different chemicals tested for induction of micronuclei (MN) in 5 different cell types across 12 different laboratories. All 14 chemicals induced biologically and statistically significant increases in MN frequency in the different cell types (L5178Y, TK6, CHO, CHL, V79) in the absence of cytochalasin B at or below target range toxicity (55+5%) irrespective of whether relative cell count (RCC), relative increase in cell count (RICC) or relative population doubling (RPD) was used as a measure of cytotoxicity/cytostasis to select the top concentration. All measures of cytotoxicity in the absence of cytochalasin B are therefore considered equally acceptable for use, and the responses were comparable to those obtained in the presence of cytochalasin B.
本文总结了在 12 个不同实验室的 5 种不同细胞类型中测试的 14 种不同化学物质的诱导微核（MN）的数据。所有 14 种化学物质在没有细胞松弛素 B 的情况下，在低于或低于目标范围毒性（55+5%）的情况下，在不同的细胞类型（L5178Y、TK6、CHO、CHL、V79）中诱导 MN 频率出现生物学和统计学上的显著增加，无论相对细胞计数（RCC）、细胞计数相对增加（RICC）还是相对群体倍增（RPD）用作细胞毒性/细胞抑制的测量标准来选择最高浓度。因此，在没有细胞松弛素 B 的情况下，所有细胞毒性测量都被认为是同样可以接受的，并且这些反应与在存在细胞松弛素 B 的情况下获得的反应相当。
