Instituto Lauro de Souza Lima, Rodovia Comandante João Ribeiro de Barros Km 225, Bauru, Brazil.
Int J Infect Dis. 2010 Sep;14 Suppl 3:e49-53. doi: 10.1016/j.ijid.2009.11.003. Epub 2010 Feb 10.
Procedures involving the use of Mycobacterium leprae and Lacazia loboi, uncultivated organisms, depend on the collection of material from the lesions of patients or experimental animals. This study compared fine-needle aspiration (FNA) and skin biopsy methods for obtaining bacilli and fungal cells to experimentally infect animals.
Lepromas from one armadillo and one enlarged footpad of a mouse previously inoculated with L. loboi were submitted to FNA and biopsy. Materials collected were processed for inoculation in mice.
Acid-fast bacilli (AFB) collected by two FNA procedures yielded 7.2×10(7) and 5.3×10(6) AFB/ml and biopsies yielded 1.58×10(8) and 3.5×10(8) AFB/ml from each leproma. Yeast-like cells of L. loboi collected by FNA yielded 1.0×10(6) fungal cells/ml and biopsy 1.0×10(7) fungal cells/ml. After 8 months, inoculated animals were sacrificed and the inoculated footpads submitted to histopathological examination and counting of AFB and fungal cells. The results obtained by the two methods were comparable for both microorganisms.
Biopsy may be replaced by FNA during harvesting of material for different purposes, especially for experimental inoculation of mice in leprosy and Jorge Lobo's disease, with the advantage of FNA being a simpler, less invasive, and less costly method.
涉及使用未经培养的分枝杆菌和拉卡兹氏利什曼原虫的程序依赖于从患者或实验动物的病变中收集材料。本研究比较了细针抽吸（FNA）和皮肤活检方法，以获取杆菌和真菌细胞来实验性感染动物。
来自一只犰狳和一只先前接种拉卡兹氏利什曼原虫的小鼠的肿大足垫的麻风结节，分别进行 FNA 和活检。收集的材料用于接种小鼠。
两种 FNA 程序采集的抗酸杆菌（AFB）分别产生 7.2×10（7）和 5.3×10（6）AFB/ml，活检分别产生 1.58×10（8）和 3.5×10（8）AFB/ml。从每个麻风结节采集的拉卡兹氏利什曼原虫酵母样细胞，FNA 产生 1.0×10（6）个真菌细胞/ml，活检产生 1.0×10（7）个真菌细胞/ml。8 个月后，接种动物被处死，接种的足垫进行组织病理学检查和 AFB 和真菌细胞计数。两种方法获得的结果对两种微生物均具有可比性。
在为不同目的采集材料时，活检可以被 FNA 取代，特别是在麻风病和豪焦氏病的小鼠实验接种中，FNA 的优点是更简单、侵入性更小且成本更低。
