Chromatofocusing.

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超能文献

作者信息

Mohammad Jamil

出版信息

Cold Spring Harb Protoc. 2010 Feb;2010(2):pdb.top67. doi: 10.1101/pdb.top67.

DOI:10.1101/pdb.top67

PMID:20150163

Abstract

摘要

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相似文献

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Cold Spring Harb Protoc. 2010 Feb;2010(2):pdb.top67. doi: 10.1101/pdb.top67.

Cleaning, regeneration, and storage of chromatofocusing columns.

Cold Spring Harb Protoc. 2010 Feb;2010(2):pdb.prot5379. doi: 10.1101/pdb.prot5379.

Preparation of the sample for chromatofocusing: buffer exchange and desalting.

Cold Spring Harb Protoc. 2010 Feb;2010(2):pdb.prot5376. doi: 10.1101/pdb.prot5376.

Removal of elution buffer from protein sample fractions after chromatofocusing.

Cold Spring Harb Protoc. 2010 Feb;2010(2):pdb.prot5378. doi: 10.1101/pdb.prot5378.

Purification of proteins by chromatofocusing.

Cold Spring Harb Protoc. 2010 Feb;2010(2):pdb.prot5377. doi: 10.1101/pdb.prot5377.

Isoelectric focusing.等电聚焦

Methods Mol Biol. 1996;59:239-48. doi: 10.1385/0-89603-336-8:239.

Development of chromatofocusing techniques employing mixed-mode column packings for protein separations.开发采用混合模式柱填料的聚焦色谱技术用于蛋白质分离。

J Chromatogr A. 2014 Jan 3;1323:57-65. doi: 10.1016/j.chroma.2013.10.090. Epub 2013 Nov 6.

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Cold Spring Harb Protoc. 2010 Feb;2010(2):pdb.prot5375. doi: 10.1101/pdb.prot5375.

Isoelectric focusing: pH gradients established with simple buffers and a cation-selective membrane.等电聚焦：用简单缓冲液和阳离子选择性膜建立pH梯度。

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