ENT Department, University Hospital, Theodor-Stern-Kai 7, 60590 Frankfurt/Main, Germany.
Anticancer Res. 2010 Jan;30(1):123-7.
BACKGROUND/AIM: Comedication with glucocorticoids such as dexamethasone is frequently given to head and neck cancer patients treated with chemotherapy. However, an increasing body of evidence suggests that dexamethasone may induce resistance to antineoplastic agents. The present study was the first to investigate the effect of dexamethasone on the antiproliferative activity of cisplatin and docetaxel in vitro in squamous cell carcinoma of the head and neck (SCCHN) cell lines.
The cytotoxic effect of cisplatin and docetaxel on eight SCCHN cell lines was determined for each drug alone or with increasing concentrations of dexamethasone. Cell growth inhibition and viability were measured quantitatively after 24, 48, 72 hours of treatment using water-soluble-tetrazolium-test and lactate dehydrogenase assays. Absolute tumor cell numbers were determined by cell counting in a Rosenthal chamber.
Cisplatin and docetaxel alone inhibited the growth of all eight SCCHN cell lines significantly (p=0.012). The antiproliferative activity of both agents was not decreased by the addition of dexamethasone in any of the cell lines (p>0.05).
Dexamethasone does not interfere with the cytotoxic action of cisplatin or docetaxel in the investigated SCCHN cell lines.
背景/目的：接受化疗的头颈部癌症患者常联合使用糖皮质激素（如地塞米松）进行治疗。然而，越来越多的证据表明，地塞米松可能会诱导肿瘤耐药。本研究首次探讨了地塞米松对体外头颈部鳞状细胞癌（SCCHN）细胞系中顺铂和多西他赛的抗增殖活性的影响。
单独使用顺铂和多西他赛以及递增浓度的地塞米松处理 8 种 SCCHN 细胞系，确定每种药物的细胞毒性作用。使用水溶性四唑盐试验和乳酸脱氢酶测定法，在 24、48、72 小时的治疗后定量测量细胞生长抑制和活力。通过罗氏 chambers 中的细胞计数确定绝对肿瘤细胞数量。
顺铂和多西他赛单独处理可显著抑制所有 8 种 SCCHN 细胞系的生长（p=0.012）。在任何细胞系中，地塞米松的添加均未降低两种药物的抗增殖活性（p>0.05）。
地塞米松不会干扰研究中 SCCHN 细胞系中顺铂或多西他赛的细胞毒性作用。
