急性冠脉综合征中8-异前列腺素F2α水平升高与全身及局部血小板活化相关。
Elevated levels of 8-iso-prostaglandin F2alpha in acute coronary syndromes are associated with systemic and local platelet activation.
作者信息
Szułdrzyński Konstanty, Zalewski Jarosław, Machnik Andrzej, Zmudka Krzysztof
机构信息
2nd Department of Medicine, Jagiellonian University School of Medicine, Kraków, Poland.
出版信息
Pol Arch Med Wewn. 2010;120(1-2):19-24.
INTRODUCTION
Oxidative stress is an important causative factor in atherosclerosis. Isoprostanes are derivatives of arachidonate oxidized by reactive oxygen species (ROS). Oxidized lipids are markers of oxidative stress, important mediators of atherosclerosis, and activators of platelets. 8-iso-prostaglandin F2alpha (8-iso-PGF2alpha) is a stable isoprostane and reliable marker of oxidative stress in vivo.
OBJECTIVES
The aim of the study was to determine the level of oxidative stress in acute coronary syndromes (ACS) and its correlations with the para meters of hemo stasis.
PATIENTS AND METHODS
Fourty-nine patients aged 46 to 76 years, including 28 with ACS and 25 with stable coronary artery disease (CAD), were enrolled to the study. The levels of 8-iso-PGF2alpha, soluble CD40 ligand (sCD40L), P-selectin (P-sel), beta-thromboglobulin, and the thrombin-antithrombin complex (TAT) in the plasma of venous blood were determined. A microvascular injury model was also used to evaluate TAT generation and sCD40L levels in blood collected every 60 seconds at the site of standardized microvascular injury.
RESULTS
8-iso-PGF2alpha levels were significantly higher in ACS compared to CAD patients (363.2 +/-45.94 vs. 328.2 -/+31.96 pg/ml, P = 0.011) and correlated with venous plasma levels of P-sel and beta-thromboglobulin in the ACS (r = 0.66; P = 0.0005 and r = 0.62; P = 0.001, respectively) and CAD groups (r = 0.46; P = 0.02 and r = 0.49; P = 0.01, respectively). In the microvascular injury model, the maximum concentrations of sCD40L in the ACS group were associated with plasma 8-iso-PGF2alpha levels (r = 0.50, P = 0.01). No correlations between 8-iso-PGF2alpha and markers of thrombin generation in venous blood and microvascular injury model were observed.
CONCLUSIONS
Plasma levels of 8-iso-PGF2alpha are significantly higher in ACS compared with stable CAD and correlate with platelet activation.
引言
氧化应激是动脉粥样硬化的一个重要致病因素。异前列腺素是花生四烯酸被活性氧（ROS）氧化后的衍生物。氧化脂质是氧化应激的标志物、动脉粥样硬化的重要介质以及血小板的激活剂。8-异前列腺素F2α（8-iso-PGF2α）是一种稳定的异前列腺素，是体内氧化应激的可靠标志物。
目的
本研究旨在确定急性冠状动脉综合征（ACS）中的氧化应激水平及其与止血参数的相关性。
患者与方法
49例年龄在46至76岁之间的患者纳入研究，其中28例患有ACS，25例患有稳定型冠状动脉疾病（CAD）。测定静脉血血浆中8-iso-PGF2α、可溶性CD40配体（sCD40L）、P-选择素（P-sel）、β-血小板球蛋白和凝血酶-抗凝血酶复合物（TAT）的水平。还使用微血管损伤模型评估在标准化微血管损伤部位每60秒采集的血液中TAT的生成和sCD40L水平。
结果
与CAD患者相比，ACS患者的8-iso-PGF2α水平显著更高（363.2±45.94 vs. 328.2±31.96 pg/ml，P = 0.011），并且与ACS组（r = 0.66；P = 0.0005）和CAD组（r = 0.46；P = 0.02）中静脉血浆P-sel和β-血小板球蛋白水平相关（分别为r = 0.62；P = 0.001和r = 0.49；P = 0.01）。在微血管损伤模型中，ACS组中sCD40L的最大浓度与血浆8-iso-PGF2α水平相关（r = 0.50，P = 0.01）。未观察到8-iso-PGF2α与静脉血和微血管损伤模型中凝血酶生成标志物之间的相关性。
结论
与稳定型CAD相比，ACS患者血浆中8-iso-PGF2α水平显著更高，且与血小板活化相关。
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