Xu Tongjiang, Ye Fei, Wang Baofeng, Tian Chunlei, Wang Sheng, Shu Kai, Guo Dongsheng, Lei Ting
Department of Neurosurgery, Tongji HospitalTongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Neuro Endocrinol Lett. 2010;31(1):147-54.
The purpose of this study was to investigate the relationship between the ghrelin or GHSR-1a mRNA levels and clinical characteristics and to confirm the effect of gsp mutations on ghrelin/GHSR-1a system in human GH-secreting pituitary adenomas.
The expression levels of ghrelin, GHSR-1a mRNA were determined by SYBR green real-time fluorescent quantitative PCR. The gsp mutations in 43 cases of human GH-secreting pituitary adenomas were detected using PCR-DNA direct sequencing analysis. Clinical data were obtained from the medical records of 43 acromegalic patients who had GH and IGF-1 assays in the same laboratory.
The expression of GHSR-1a correlated positvely with tumor size (R=0.411, p=0.006) and invasiveness (p<0.05). In contrast, ghrelin mRNA levels correlated positively only with tumor size (R=0.331, p=0.030) but not with tumor invasiveness (p>0.05). The expression level of GHSR-1a mRNA was significantly higher in gsp positive adenomas than in negative adenomas (p<0.05). Whereas, there was no significant difference in the expression of ghrelin mRNA between gsp mutation-positive and -negative adenomas (p>0.05). Additionally there was a significant positve correlation between the ghrelin and GHSR-1a mRNA expression levels in gsp-positive (R=0.553, p=0.040) or -negative adenomas (R=0.489, p=0.007).
GHSR-1a correlated positively with tumor size and invasiveness, while ghrelin correlated positively only with tumor size. Gsp mutations may upregulate the expression of GHSR-1a mRNA and have no effect on ghrelin mRNA levels in human GH-secreting pituitary adenomas.
本研究旨在探讨胃饥饿素或GHSR-1a mRNA水平与临床特征之间的关系，并证实gsp突变对人分泌生长激素垂体腺瘤中胃饥饿素/GHSR-1a系统的影响。
采用SYBR green实时荧光定量PCR法测定胃饥饿素、GHSR-1a mRNA的表达水平。利用PCR-DNA直接测序分析检测43例人分泌生长激素垂体腺瘤中的gsp突变。临床数据来自43例在同一实验室进行生长激素和胰岛素样生长因子-1检测的肢端肥大症患者的病历。
GHSR-1a的表达与肿瘤大小呈正相关（R=0.411，p=0.006），与侵袭性呈正相关（p<0.05）。相比之下，胃饥饿素mRNA水平仅与肿瘤大小呈正相关（R=0.331，p=0.030），而与肿瘤侵袭性无关（p>0.05）。gsp阳性腺瘤中GHSR-1a mRNA的表达水平显著高于阴性腺瘤（p<0.05）。然而，gsp突变阳性和阴性腺瘤之间胃饥饿素mRNA的表达无显著差异（p>0.05）。此外，gsp阳性（R=0.553，p=0.040）或阴性腺瘤（R=0.489，p=0.007）中胃饥饿素和GHSR-1a mRNA表达水平之间存在显著正相关。
GHSR-1a与肿瘤大小和侵袭性呈正相关，而胃饥饿素仅与肿瘤大小呈正相关。gsp突变可能上调人分泌生长激素垂体腺瘤中GHSR-1a mRNA的表达，而对胃饥饿素mRNA水平无影响。
