Analytical Division, Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India.
Anal Chim Acta. 2010 Mar 3;662(1):14-22. doi: 10.1016/j.aca.2009.12.037. Epub 2010 Jan 4.
A simple polymerization strategy is reported in this work which allows molecularly imprinted polymeric fiber (monolith) fabrication for direct use in sensing devices. This is advantageous for achieving higher degree of enrichment of target analyte (folic acid) from the complex matrices of real samples, without any surface fouling, cross-reactivity, and non-specific (false-positive) contributions. In order to measure serum folic acid at ultratrace level to detect spina bifida, a neural tube defect in mother, and other acute cases of proteomic diseases, the hyphenation between molecularly imprinted micro-solid phase extraction fiber and a complementary molecularly imprinted polymer-carbon composite fiber sensor has been found quite efficient. The primitive diagnosis of many chronic diseases is feasible by estimating folic acid as biomarker, with the detection limit as low as 0.0036 ng mL(-1) (relative standard deviation=0.13%, signal/noise=3) in human blood serum.
本工作报道了一种简单的聚合策略，该策略允许制备分子印迹聚合物纤维（整体柱），可直接用于传感装置。这有利于从复杂的实际样品基质中实现更高程度的目标分析物（叶酸）的富集，而不会出现任何表面污垢、交叉反应和非特异性（假阳性）的影响。为了在痕量水平下测量血清叶酸以检测脊柱裂，一种神经管缺陷疾病，以及其他蛋白质组疾病的急性病例，已经发现分子印迹微固相萃取纤维与互补的分子印迹聚合物-碳复合纤维传感器之间的连接非常有效。通过将叶酸作为生物标志物进行估计，可以实现许多慢性疾病的初步诊断，在人血清中的检测限低至 0.0036ng/mL（相对标准偏差=0.13%，信号/噪声=3）。
