Au nanoparticle decorated silicate network for the amperometric sensing of isoniazid.

Au nanoparticle (nAu) based electrochemical platform for the amperometric sensing of isoniazid at sub-nanomolar level is developed. The sol-gel derived 3-dimensional silicate network pre-assembled on a conducting substrate is chemically decorated with nAu of 70-100 nm by seed-mediated growth approach. The Au nanoseeds are first chemisorbed onto the thiol functional groups of the silicate network and their size was enlarged by hydroxylamine seeding. The nanoparticles efficiently catalyze the oxidation of isoniazid at less positive potential. Large decrease in the overpotential and significant enhancement in the anodic peak current with respect to the polycrystalline Au electrode are observed. The nanoparticle based platform is highly sensitive (4.03+/-0.01 nA/nM) and it linearly responds to isoniazid up to the concentration of 1mM. It could detect as low as 0.1 nM (S/N=5) of isoniazid at the potential of 10 mV in aqueous solution without any redox mediator. The catalytic response of the sensing platform depends on the amount of nanoparticles loaded onto the silicate network. Very interestingly, the sensing platform could simultaneously detect isoniazid and hydrazine in their coexistence without compromising the sensitivity. Well separated individual voltammetric response is obtained for both analytes. The sensing platform is highly stable and it can be repeatedly used for 7 days.