School of Chemistry and Chemical Engineering, Sun Yat-sen University, 135 Xingang Xi Road, Guangzhou 510275, China.
Talanta. 2010 Mar 15;80(5):2099-105. doi: 10.1016/j.talanta.2009.11.015. Epub 2009 Nov 13.
The combination of molecular imprinting and solid-phase microextraction (SPME) technique provides a powerful sample preparation tool in terms of selectivity, simplicity, and flexibility. This paper reports a novel molecularly imprinted polymer (MIP) coated SPME fiber with 17beta-estradiol as template by improved multiple co-polymerization method. The obtained fiber exhibits excellent characteristics such as high porosity, good thermal and chemical stability. Extraction performance shows that the MIP-coated fiber has stronger affinity to the template molecule as compared with the commercial SPME fibers and the control polymer-coated fiber without addition of template. Owing to the shape and structural compatibility, the obtained fiber also demonstrated specific selectivity to the structural related compounds of 17beta-estradiol, such as estriol, estrone and 17 alpha-ethynylestradiol, and thus can be applied to simultaneous determination of these estrogens from complex samples coupled with high performance liquid chromatography. The variables that influence extraction were investigated. The MIP-coated fiber demonstrated its efficiency for extraction of estrogens in fishery samples. The detection limits were in the range of 0.98-2.39 microg L(-1), and the recoveries were 80.0-83.6% and 85.0-94.1% for fish and shrimp tissue samples, respectively.
分子印迹与固相微萃取（SPME）技术的结合在选择性、简单性和灵活性方面提供了一种强大的样品制备工具。本文报道了一种新型的分子印迹聚合物（MIP）涂层 SPME 纤维，以 17β-雌二醇为模板，采用改进的多共聚法制备。所得纤维具有高孔隙率、良好的热稳定性和化学稳定性等特点。萃取性能表明，与商业 SPME 纤维和未添加模板的对照聚合物涂层纤维相比，MIP 涂层纤维对模板分子具有更强的亲和力。由于形状和结构的兼容性，所得纤维还对 17β-雌二醇的结构相关化合物，如雌三醇、雌酮和 17α-乙炔基雌二醇具有特异性选择性，因此可以与高效液相色谱法结合，用于从复杂样品中同时测定这些雌激素。考察了影响萃取的变量。MIP 涂层纤维在渔业样品中萃取雌激素的效率很高。检测限在 0.98-2.39μg/L 范围内，鱼和虾组织样品的回收率分别为 80.0-83.6%和 85.0-94.1%。
