Key Laboratory of Eco-Chemical Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, People's Republic of China.
Talanta. 2010 Mar 15;80(5):2177-81. doi: 10.1016/j.talanta.2009.11.026. Epub 2009 Nov 14.
In this paper a carbon ionic liquid electrode (CILE) was fabricated by using ionic liquid 1-ethyl-3-methylimidazolium ethylsulfate ([EMIM]EtOSO(3)) as modifier and further gold nanoparticles were in situ electrodeposited on the surface of CILE. The fabricated Au/CILE was used as a new platform for the immobilization of hemoglobin (Hb) with the help of a Nafion film. Electrochemical experimental results indicated that direct electron transfer of Hb was realized on the surface of Au/CILE with a pair of well-defined quasi-reversible redox peaks appeared. The formal peak potential (E(0)') was obtained as -0.210 V (vs. SCE) in pH 7.0 phosphate buffer solution (PBS), which was the characteristic of Hb heme Fe(III)/Fe(II) redox couple. The fabricated Nafion/Hb/Au/CILE showed excellent electrocatalytic activity to the reduction of trichloroacetic acid (TCA) and the reduction peak current was in proportional to TCA concentration in the range from 0.2 to 18.0 mmol/L with the detection limit as 0.16 mmol/L (S/N=3). The proposed electrode showed good stability and reproducibility, and it had the potential application as a new third-generation electrochemical biosensor.
本文采用离子液体 1-乙基-3-甲基咪唑硫酸乙酯([EMIM]EtOSO(3))作为修饰剂制备了碳离子液体电极(CILE)，并进一步在 CILE 表面原位电沉积了金纳米粒子。所制备的 Au/CILE 借助 Nafion 膜作为血红蛋白(Hb)固定的新平台。电化学实验结果表明，Hb 在 Au/CILE 表面实现了直接电子转移，出现了一对良好定义的准可逆氧化还原峰。在 pH 7.0 磷酸盐缓冲溶液(PBS)中，获得的形式峰电位(E(0)')为-0.210 V(vs. SCE)，这是 Hb 血红素 Fe(III)/Fe(II)氧化还原对的特征。所制备的 Nafion/Hb/Au/CILE 对三氯乙酸(TCA)的还原表现出优异的电催化活性，还原峰电流与 TCA 浓度在 0.2 至 18.0 mmol/L 范围内呈正比，检测限为 0.16 mmol/L(S/N=3)。该电极具有良好的稳定性和重现性，有望作为新型第三代电化学生物传感器得到应用。
