Department of Molecular Biophysics, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland.
Talanta. 2010 Mar 15;80(5):2196-8. doi: 10.1016/j.talanta.2009.11.004. Epub 2009 Nov 10.
Peroxynitrite and hypochlorite are oxidants relevant in many pathological situations. We propose a simple spectrofluorometric assay to determine antioxidant capacity against hypochlorite and peroxynitrite based on protection against fluorescein bleaching. The assay can be performed on a microplate and requires minute amounts of material. Standard antioxidants show different reactivities for both oxidants. Antioxidant capacity of fruit bodies of edible mushrooms, tea, coffee and wine estimated as exemplary biological material point to considerable differences in the scavenging capacity of various biological material against hypochlorite, peroxynitrite and peroxyl radicals.
过氧亚硝酸盐和次氯酸盐是许多病理情况下相关的氧化剂。我们提出了一种简单的荧光光度法测定抗次氯酸盐和过氧亚硝酸盐的抗氧化能力的方法，该方法基于对抗荧光素漂白的保护作用。该测定方法可在微孔板上进行，所需材料量极少。标准抗氧化剂对两种氧化剂的反应性不同。以可食用蘑菇、茶、咖啡和酒的子实体作为典型生物材料来估计其抗氧化能力，结果表明，各种生物材料对次氯酸盐、过氧亚硝酸盐和过氧自由基的清除能力存在显著差异。
