用链霉亲和素标记的量子点荧光免疫分析法测定 17β-雌二醇。

Determination of 17beta-oestradiol by fluorescence immunoassay with streptavidin-conjugated quantum dots as label.

机构信息

College of Chemistry and Chemical Engineering, Liaocheng University, No. 1 Hunan Road, Liaocheng, Shandong 252059, PR China.

出版信息

Steroids. 2010 Jun;75(6):400-3. doi: 10.1016/j.steroids.2010.02.002. Epub 2010 Feb 10.

DOI:10.1016/j.steroids.2010.02.002

PMID:20152848

Abstract

A sensitive and selective method for the determination of 17beta-oestradiol by fluorescence immunoassay (FIA) was established on the basis of quantum dots (QDs) as label. The complex of biotin-labelled anti-rabbit IgG and strepavidin conjugated by quantum dots (QD-SA) was regarded as a probe in this system and the strepavidin-biotin system as signal amplification system. After optimising the conditions of the immunoreaction, such as the concentration of the reagent and the pH of the buffer solution, the linear range and the limit of detection of 17beta-oestradiol were 0.01-10,000ngml(-1) and 0.00542ngml(-1), respectively. This method was applied to determine oestradiol in water samples, with the percent recoveries in the range of 86-113%.

摘要

建立了一种基于量子点（QDs）作为标记的用于测定 17β-雌二醇的荧光免疫分析（FIA）的灵敏和选择性方法。在该体系中，将生物素标记的抗兔 IgG 与量子点偶联的链霉亲和素（QD-SA）复合物用作探针，并将链霉亲和素-生物素系统用作信号放大系统。在优化免疫反应条件（如试剂浓度和缓冲溶液的 pH 值）后，17β-雌二醇的线性范围和检测限分别为 0.01-10,000ngml（-1）和 0.00542ngml（-1）。该方法用于测定水样中的雌二醇，回收率在 86-113%范围内。