Croucher Institute for Environmental Sciences, and Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong, PR China.
Ecotoxicol Environ Saf. 2010 May;73(4):541-9. doi: 10.1016/j.ecoenv.2010.01.012. Epub 2010 Feb 11.
Sediments from Mai Po Ramsar site, Hong Kong were in general shown to be highly toxic based on the results of four toxicity tests (Microtox solid-phase test, Daphnia mortality test, algal [Microcystis aeruginosa] growth inhibition test and ryegrass [Lolium perenne] seed germination/root elongation test). Sediment of the mudflat (which is open to Deep Bay, i.e., the pollution source) was the most toxic while sediment of gei wai 24g (an enclosed freshwater pond) was the least toxic. Results of biomarker studies (tilapia hepatic metallothionein; glutathione (GSH) and EROD activity using H4IIE rat hepatoma cell) were also concordant with those in the toxicity tests. Significant liner relationships (p<0.01) were found between GSH contents in the rat hepatoma cells and PAHs, OCPs contents in the sediment extracts. It is recommended that the present suite of bioassays is useful and is biologically relevant for future ecotoxicological studies focusing on similar wetlands.
香港米埔拉姆萨尔湿地的沉积物在四项毒性测试（Microtox 固相测试、溞类死亡率测试、藻类（铜绿微囊藻）生长抑制测试和黑麦草种子发芽/根伸长测试）的结果表明，总体上具有高度毒性。泥滩的沉积物（位于向大亚湾开放的区域，即污染源）毒性最大，而围内 24g 池塘（一个封闭的淡水池塘）的沉积物毒性最小。生物标志物研究（罗非鱼肝金属硫蛋白；使用 H4IIE 大鼠肝癌细胞的谷胱甘肽 (GSH) 和 EROD 活性）的结果也与毒性测试的结果一致。在大鼠肝癌细胞中的 GSH 含量与沉积物提取物中的多环芳烃 (PAHs) 和有机氯农药 (OCPs) 含量之间存在显著的线性关系（p<0.01）。建议未来在开展类似湿地的生态毒理学研究时，采用本套生物测定方法作为有用且具有生物学相关性的工具。
