Eggermont J A, Wuytack F, Casteels R
Laboratorium voor Fysiologie, KU Leuven, Belgium.
Biochim Biophys Acta. 1991 Mar 26;1088(3):448-51. doi: 10.1016/0167-4781(91)90143-a.
The gene 2 for the sarcoplasmic/endoplasmic-reticulum Ca2+ pump (SERCA2) is expressed both in muscle and non-muscle tissues and four distinct SERCA2 mRNAs which differ only in their 3' end have been described. In order to understand the differential 3' end processing of the SERCA2 transcripts, we have now cloned a 12.6 kb pig genomic clone which contains the 3' end of the pig SERCA2 gene. Part of this clone (7510 nucleotides) was sequenced and it was found to contain four constitutive exons followed by four optional exons which underlie the 3' end diversity of the SERCA2 mRNAs. The inclusion or the exclusion of these optional exons is explained by the presence of four optional processing sites: two polyadenylation sites and two optional donor splice sites.
肌浆网/内质网Ca2+泵（SERCA2）的基因2在肌肉和非肌肉组织中均有表达，并且已经描述了四种仅在3'端不同的不同SERCA2 mRNA。为了了解SERCA2转录本的差异3'端加工过程，我们现在克隆了一个12.6 kb的猪基因组克隆，该克隆包含猪SERCA2基因的3'端。对该克隆的一部分（7510个核苷酸）进行了测序，发现它包含四个组成型外显子，随后是四个选择性外显子，这些外显子构成了SERCA2 mRNA的3'端多样性。这些选择性外显子的包含或排除可以通过四个选择性加工位点来解释：两个聚腺苷酸化位点和两个选择性供体剪接位点。
