Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
Phytomedicine. 2010 Jul;17(8-9):659-68. doi: 10.1016/j.phymed.2009.10.010. Epub 2010 Feb 11.
In order to research the target of superior efficacy and lesser side effects, combination of herbal materials has been applied to phytotherapy for thousands of years in China and some other countries. Zuojin Wan (ZJW), a famous traditional Chinese medicine formula, is used in treating gastric diseases in China. It is composed of two herbs, Rhizoma Coptidis (RC) and Fructus Evodiae (FE) in the ratio of 6: 1(w/w). In the present study, we examined the effects of ZJW, RC, FE and active components isolated from these herbs on catecholamine (CA) secretion and intracellular calcium (Ca(2+)) in cultured bovine adrenal medullary cells. Extracts of ZJW and RC and berberine, palmatine and jatrorrhizine, components of RC, all inhibited CA secretion and rise in Ca(2+) induced by acetylcholine (ACh), veratridine (Ver) and/or 56 mM K(+). On the other hand, extract of FE, evodiamine and rutaecarpine, components of FE, stimulated CA secretion and rise in Ca(2+) induced by ACh. Furthermore, different proportions of RC and FE caused different responses in CA secretion. The present findings suggest that two herbs in ZJW have opposite effects, i.e., inhibitory effect of RC and stimulatory effect of FE, on CA secretion induced by acetylcholine in cultured bovine adrenal medullary cells.
为了研究高效低副作用的目标，草药的组合已被应用于中国和其他一些国家的植物疗法几千年。左金丸（ZJW），一种著名的中药配方，在中国用于治疗胃病。它由两种草药组成，黄连（RC）和吴茱萸（FE），比例为 6:1（w/w）。在本研究中，我们研究了 ZJW、RC、FE 的提取物以及从这些草药中分离的活性成分对培养的牛肾上腺髓质细胞儿茶酚胺（CA）分泌和细胞内钙离子浓度（[Ca （2+）]（i））的影响。ZJW 和 RC 的提取物以及黄连素、巴马汀和小檗碱，RC 的成分，均抑制了乙酰胆碱（ACh）、藜芦碱（Ver）和/或 56mM K +诱导的 CA 分泌和[Ca （2+）]（i）的升高。另一方面，FE 的提取物、吴茱萸碱和吴茱萸卡品，FE 的成分，刺激了 ACh 诱导的 CA 分泌和[Ca （2+）]（i）的升高。此外，RC 和 FE 的不同比例对 CA 分泌产生了不同的反应。本研究结果表明，ZJW 中的两种草药对乙酰胆碱诱导的培养牛肾上腺髓质细胞 CA 分泌具有相反的作用，即 RC 的抑制作用和 FE 的刺激作用。
