Dipartimento di Chimica e Tecnologie Farmaceutiche, via Archirafi 32, 90123 Palermo, Italy.
Int J Pharm. 2010 May 10;390(2):183-90. doi: 10.1016/j.ijpharm.2010.02.002. Epub 2010 Feb 11.
Poly[monomethylnona(ethylene glycol) 1-methylene-3-(4-methylphenyl)-1H-indene-2-carboxylate] (poly-1b) a new polymer based on a PEG-functionalized benzofulvene macromonomer have been investigated as hydrogel-based material for complexation and release of immunoglobulin (IgG) at physiological mimicking conditions. The polymer ability to complex human IgG has been studied by preparing copolymer/protein complexes obtained by spontaneous protein interactions onto polymer hydrogel aggregates, and the protein release rate has been evaluated at physiological conditions. SEM analysis was used to visualize the copolymer/IgG aggregates and its microstructured deposition. Moreover, rheological studies performed at 37 degrees C allowed determining hydrogel mechanical properties. On the basis of these information and NMR transverse relaxation measurements, the estimation of hydrogel mesh size distribution was possible. Finally, biological studies performed with poly-1b aqueous dispersions showed no cytotoxic effect on MCF-7 cell line, suggesting potential biocompatibility features for this polymer and making this new polymer a good potential candidate for the production of drug delivery systems.
聚[单甲基壬（乙二醇）1-亚甲基-3-（4-甲基苯基）-1H-茚-2-羧酸酯]（聚-1b）是一种基于聚乙二醇（PEG）功能化苯并呋喃大分子单体的新型聚合物，已被研究作为水凝胶基材料，用于在生理模拟条件下络合和释放免疫球蛋白（IgG）。通过制备通过聚合物水凝胶聚集体上的自发蛋白质相互作用获得的共聚物/蛋白质复合物，研究了聚合物与人类 IgG 络合的能力，并在生理条件下评估了蛋白质释放速率。SEM 分析用于可视化共聚物/IgG 聚集体及其微结构沉积。此外，在 37 摄氏度下进行的流变学研究允许确定水凝胶的机械性能。基于这些信息和 NMR 横向弛豫测量，可估算水凝胶的网格尺寸分布。最后，用聚-1b 水分散体进行的生物学研究表明，对 MCF-7 细胞系没有细胞毒性作用，表明该聚合物具有潜在的生物相容性特征，使这种新型聚合物成为生产药物输送系统的良好候选材料。
