Mechanistic Studies Division, Environmental Health Science and Research Bureau, Health Canada, Ottawa K1A0K9, Canada.
J Chromatogr A. 2010 May 7;1217(19):3269-74. doi: 10.1016/j.chroma.2010.01.048. Epub 2010 Jan 25.
Oxidative stress has been implicated in various pathologies as well as in environmental pollutant-induced negative health outcomes. In this work we have developed an analytical method to measure oxidative stress markers namely m-, o-tyrosine, 3-chlorotyrosine, 3-nitrotyrosine, and the DNA damage marker 8-hydroxy deoxyguanosine in human urine. The method involves the base hydrolysis of the urine sample followed by solid phase extraction of target analytes using a reverse phase polymeric sorbent column prior to the HPLC-EC analysis. The recovery studies indicated that the overall method recovery for all analytes were >60%. The limit of quantification of all target analytes was <10nM with a linear range from 2 nM to 150 microM. The applicability of this method is demonstrated by analyzing the above markers in healthy human urine (n=10) samples.
氧化应激与各种病理以及环境污染物引起的负面健康后果有关。在这项工作中，我们开发了一种分析方法来测量氧化应激标志物，即 m-、o-酪氨酸、3-氯酪氨酸、3-硝基酪氨酸和 DNA 损伤标志物 8-羟基脱氧鸟苷在人尿中的含量。该方法包括尿液样品的碱水解，然后使用反相聚合物吸附剂柱对目标分析物进行固相萃取，再进行 HPLC-EC 分析。回收研究表明，所有分析物的总方法回收率均>60%。所有目标分析物的定量下限均<10nM，线性范围为 2nM 至 150μM。通过分析 10 名健康人尿液样本中的上述标志物，证明了该方法的适用性。
