Hong L, Mulholland J, Chinsky J M, Knudsen T B, Kellems R E, Glasser S R
Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030.
Biol Reprod. 1991 Jan;44(1):83-93. doi: 10.1095/biolreprod44.1.83.
Adenosine deaminase (ADA) is expressed in high concentrations at the fetal-maternal interface during postimplantation stages of gestation in the mouse. The experiments reported here were designed to identify the specific uterine cells that express ADA subsequent to implantation in the rat and to determine if embryonic cells contribute to ADA expression. The results of biochemical analysis demonstrate that ADA-specific activity increases to very high levels in implantation sites, beginning approximately 72 h after blastocyst attachment. Immunocytochemical analysis localized this ADA expression to the decidualized stromal cells in the antimesometrial region of the pregnant uterus. In experimentally induced deciduoma, these cells were capable of synthesizing high levels of both ADA and mRNA for ADA in the absence of embryos. The enzyme first appeared in decidual cell cytoplasm, approximately 72 h after induction of decidualization, and later was localized in the decidual cell nuclei. Since the expression of ADA and its mRNA in decidual cells follows the appearance of desmin, a protein marker for decidualization, by at least 48 h, ADA appears to be involved in the functioning of mature decidual cells rather than in stromal cell differentiation. The expression of ADA, but not desmin, was restricted to the antimesometrial decidual cells and decreased when these cells regressed. At mid-gestation ADA activity increased and was localized principally in the fetal placenta. The results presented here demonstrate that ADA is localized to the antimesometrial decidual cell and that its expression is consequent to differentiation of the uterine stromal cell and independent of any embryonic stimulus.
腺苷脱氨酶（ADA）在小鼠妊娠植入后阶段的母胎界面高浓度表达。本文报道的实验旨在确定大鼠植入后表达ADA的特定子宫细胞，并确定胚胎细胞是否有助于ADA的表达。生化分析结果表明，在胚泡附着后约72小时开始，植入部位的ADA特异性活性增加到非常高的水平。免疫细胞化学分析将这种ADA表达定位到妊娠子宫反系膜区域的蜕膜化基质细胞。在实验诱导的蜕膜瘤中，这些细胞在没有胚胎的情况下能够合成高水平的ADA和ADA的mRNA。该酶最初出现在蜕膜化诱导后约72小时的蜕膜细胞质中，随后定位于蜕膜细胞核中。由于蜕膜细胞中ADA及其mRNA的表达至少在作为蜕膜化蛋白标志物的结蛋白出现后48小时出现，因此ADA似乎参与成熟蜕膜细胞的功能，而不是基质细胞分化。ADA的表达，但不是结蛋白的表达，仅限于反系膜蜕膜细胞，并且当这些细胞退化时降低。在妊娠中期，ADA活性增加，主要定位于胎儿胎盘。本文给出的结果表明，ADA定位于反系膜蜕膜细胞，其表达是子宫基质细胞分化的结果，且独立于任何胚胎刺激。
