Central Laboratory, Shanghai Xuhui Central Hospital, 966 Huaihai Middle Road, Shanghai 200031, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Mar 15;878(9-10):758-62. doi: 10.1016/j.jchromb.2010.01.020. Epub 2010 Jan 25.
Melamine was found to be the etiological factor for the urinary stones epidemic in infants and young children in China in 2008. Urine level of melamine and its analog cyanuric acid may be useful markers for the evaluation of toxic effects. Liquid chromatography tandem mass spectrometry methods for the individual determination of melamine and cyanuric acid in human urine are described. Using isotope labeled internal standards during liquid-liquid extraction, the method was fully validated by verifying specificity, linearity, LLOQ, intra- and inter-assay precision and accuracy, matrix effect, recovery and stability. Calibration curves with good linearity (r=0.9999) over the concentration range from 10 to 5000 ng/ml, intra-assay precision <10% and inter-assay precision <15%, accuracy between 93.0 and 111.6% were obtained with multiple reaction monitoring mode for melamine and cyanuric acid in human urine. The methods were successfully applied to the analysis of urine samples collected from 86 infants and 110 adults.
三聚氰胺被发现是 2008 年中国婴幼儿尿路结石流行的病因。三聚氰胺及其类似物氰尿酸的尿水平可能是评估毒性作用的有用标志物。本文描述了用于人尿中三聚氰胺和氰尿酸的单独测定的液相色谱-串联质谱法。通过液-液萃取过程中使用同位素标记的内标，该方法通过验证特异性、线性、LLOQ、内和日间精密度和准确度、基质效应、回收率和稳定性进行了全面验证。对于人尿中的三聚氰胺和氰尿酸，采用多重反应监测模式，在 10 至 5000ng/ml 的浓度范围内获得了良好线性（r=0.9999）、<10%的日内精密度和 <15%的日间精密度、93.0%至 111.6%的准确度的校准曲线。该方法成功应用于 86 名婴幼儿和 110 名成年人尿液样本的分析。
