Tang Xiao-Shuang, Shi Xi-Yan, Tang Yu-Hai, Yue Zhong-Jin, He Qi-Qi
The Second Affiliated Hospital, Lanzhou University, Lanzhou 730030, China.
Institute of Analytical Sciences, Xi'an Jiaotong University, Xi'an 710061, China.
J Pharm Anal. 2011 May;1(2):104-107. doi: 10.1016/S2095-1779(11)70018-1. Epub 2012 Jan 30.
A sensitive chemiluminescence (CL) method was developed for determining melamine in urine and plasma samples based on the fact that melamine can remarkably enhance the chemiluminescence of Luminol-K Fe(CN) system in alkaline medium. The determination conditions were optimized. Under optimum conditions, the chemiluminescence intensity had a good linear relationship with melamine in the range of 9.0 × 10 - 7.0 × 10 g/mL with a correlation coefficient of 0.9992. The detection limits (3σ) were 3.54 ng/mL for urine sample and 6.58 ng/mL for plasma sample. The average recoveries of melamine were 102.6% for urine sample and 95.1% for plasma sample. Melamine in samples was extracted with liquid-liquid extraction procedures and the assay results coincided very well with that determined with flow injection chemiluminescence method. The method provides a reproducible and stable approach for sensitive detection and quantification of melamine in urine and plasma samples.
基于三聚氰胺能显著增强鲁米诺 - 铁氰化钾体系在碱性介质中的化学发光这一事实，开发了一种灵敏的化学发光（CL）方法用于测定尿液和血浆样品中的三聚氰胺。对测定条件进行了优化。在最佳条件下，化学发光强度与三聚氰胺在9.0×10⁻⁷.0×10⁻⁵g/mL范围内呈良好的线性关系，相关系数为0.9992。尿液样品的检测限（3σ）为3.54 ng/mL，血浆样品的检测限为6.58 ng/mL。尿液样品中三聚氰胺的平均回收率为102.6%，血浆样品中为95.1%。采用液 - 液萃取法提取样品中的三聚氰胺，测定结果与流动注射化学发光法测定结果非常吻合。该方法为灵敏检测和定量尿液及血浆样品中的三聚氰胺提供了一种可重复且稳定的方法。
