重组卡介苗亚株的分泌组揭示了假定蛋白的差异。

The secretome of a recombinant BCG substrain reveals differences in hypothetical proteins.

机构信息

Programa de Inmunología Molecular Microbiana, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Edificio de Investigación, 4 degrees piso, Av. Universidad #3000, Coyoacán, 04510 México, D.F., Mexico.

出版信息

Vaccine. 2010 May 21;28(23):3997-4001. doi: 10.1016/j.vaccine.2010.01.064. Epub 2010 Feb 12.

DOI:10.1016/j.vaccine.2010.01.064

PMID:20153797

Abstract

Tuberculosis remains a major human health problem worldwide, and strategies for its prevention include the generation and characterization of new recombinant vaccines containing immunodominant antigens from Mycobacterium tuberculosis. By comparing the secretomes of wild-type Mycobacterium bovis and a PstS1-recombinant M. bovis BCG vaccine substrain (rBCG38), we identified six conserved hypothetical proteins (BCG2696, BCG1674, BCG0372, BCG0427, BCG2436c, and BCG3053) that are differentially expressed. Our findings will aid in the identification of highly immunogenic proteins present in rBCG.

摘要

结核病仍然是全球范围内的一个主要人类健康问题，其预防策略包括生成和表征含有结核分枝杆菌免疫优势抗原的新型重组疫苗。通过比较野生型牛分枝杆菌和 PstS1 重组牛分枝杆菌卡介苗亚株（rBCG38）的分泌组，我们鉴定出六个保守的假定蛋白（BCG2696、BCG1674、BCG0372、BCG0427、BCG2436c 和 BCG3053），它们的表达存在差异。我们的发现将有助于鉴定 rBCG 中存在的高免疫原性蛋白。