Department of Biological Sciences, Auburn University, Auburn, AL 368491, USA.
Appl Environ Microbiol. 2010 Apr;76(8):2673-7. doi: 10.1128/AEM.02520-09. Epub 2010 Feb 12.
The viral metagenome within an activated sludge microbial assemblage was sampled using culture-dependent and culture-independent methods and compared to the diversity of activated sludge bacterial taxa. A total of 70 unique cultured bacterial isolates, 24 cultured bacteriophages, 829 bacterial metagenomic clones of 16S rRNA genes, and 1,161 viral metagenomic clones were subjected to a phylogenetic analysis.
采用培养依赖和非培养方法对活性污泥微生物组合中的病毒宏基因组进行了采样,并与活性污泥细菌分类群的多样性进行了比较。总共对 70 个独特的培养细菌分离物、24 个培养噬菌体、829 个细菌宏基因组 16S rRNA 基因克隆和 1161 个病毒宏基因组克隆进行了系统发育分析。