Zigova T, Graziadei P P, Monti Graziadei A G
Department of Biological Sciences, Florida State University, Tallahassee 32306.
Brain Res. 1991 Jan 18;539(1):51-8. doi: 10.1016/0006-8993(91)90685-o.
Tritiated thymidine prelabeled presumptive olfactory bulbs (E15-E17, and E19) were homotopically transplanted in unilaterally partially or totally bulbectomized neonatal rats (P1-P5). [3H]thymidine was injected to pregnant rats at the time when the large neurons of the bulb were undergoing cellular division. After postoperative survival times from 20 days to 7 months, the animals were sacrificed and processed for histological, immunohistochemical and autoradiographic observations. The nuclear autoradiographic label allowed easy recognition of the transplanted tissue in totally bulbectomized animals after short survival and in partially bulbectomized animals after long survival. The autoradiographic label was strictly confined to the transplanted tissue and intermingling of host and donor neurons was never observed. The reliability of the autoradiographic technique in our study will enable us to mark those neurons whose axons can be demonstrated, by retrograde tracing methods, to establish connections with the host brain.
将氚标记的胸腺嘧啶核苷预标记的假定嗅球（胚胎第15 - 17天和第19天）同位移植到单侧部分或完全切除嗅球的新生大鼠（出生后第1 - 5天）体内。在嗅球大神经元进行细胞分裂时，向怀孕大鼠注射[3H]胸腺嘧啶核苷。术后存活20天至7个月后，处死动物并进行组织学、免疫组织化学和放射自显影观察。核放射自显影标记使得在短期存活的完全切除嗅球的动物以及长期存活的部分切除嗅球的动物中能够轻松识别移植组织。放射自显影标记严格局限于移植组织，从未观察到宿主和供体神经元的混合。我们研究中放射自显影技术的可靠性将使我们能够标记那些其轴突可通过逆行追踪方法证明与宿主脑建立连接的神经元。
