College of Life Science, Northeast Normal University, 130024, Changchun, People's Republic of China.
Biotechnol Lett. 2010 Jun;32(6):861-6. doi: 10.1007/s10529-010-0220-1. Epub 2010 Feb 13.
A novel glycogen synthase kinase-3 gene, GmGSK, was isolated from Glycine max. It is 1,596 bp in length with one ORF of 410 amino acids. Southern blot analysis revealed that it has at least two copies in the G. max genome. GmGSK, when transiently expressed in Nicotiana tabacum leaves, was localized in both cell membrane and cytoplasm. Northern blot analysis indicated that GmGSK is expressed in all tissues, with highest expression in the root. GmGSK can be induced by various abiotic stresses. When transformed with GmGSK, Saccharomyces cerevisiae exhibited enhanced resistance to salt and drought stress.
从大豆中分离到一个新的糖原合成酶激酶基因 GmGSK，其 cDNA 全长 1596bp，含有一个 410 个氨基酸的开放阅读框。Southern blot 分析表明 GmGSK 基因在大豆基因组中至少有两个拷贝。GmGSK 在烟草叶片中转录表达时，定位于细胞膜和细胞质中。Northern blot 分析表明 GmGSK 在所有组织中都有表达，在根中表达量最高。GmGSK 可被多种非生物胁迫诱导。将 GmGSK 转入酿酒酵母后，酵母的耐盐和耐旱能力增强。
