Shou Zhexing, Shen Lin, Xiong Pengcheng
Department of Integrated Traditional Chinese and Western Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
J Huazhong Univ Sci Technolog Med Sci. 2010 Feb;30(1):85-8. doi: 10.1007/s11596-010-0115-3. Epub 2010 Feb 14.
The efficacy of injecting sclerosing agent next to transverse process of cervical vertebra to induce vertebral artery type of cervical syndrome (CSA) was observed. Twenty rabbits were randomly divided into two groups: the model group and the control group. The rabbits in the model group were injected with sclerosing agent next to transverse process of cervical vertebray, on the contrary, the rabbits in the control group were injected with nothing. Transcranial Doppler (TCD) was used to detect the average speed of blood (Vm), pulsatility index (Pi) and the resistant index (Ri) of the vertebral artery, hematoxylin and eosin (HE) staining was used to observe the morphological changes, and immunohistochemistry was used to detect the expression of alpha-smooth muscle actin (alpha-SMA) and matrix metalloproteinase-2(MMP-2). TCD showed increased Pi, Ri and decreased Vm in the model group (P<0.05) compared with the control group. HE staining revealed hyperplasia and hypertrophied smooth muscle cells in the model group (P<0.05). Immunohistochemistry displayed up-regulation of alpha-SMA and MMP-2 in the model group (P<0.05). It was concluded that injecting sclerosing agent next to transverse process of cervical vertebra induces remodeling of vertebral artery in rabbits, suggesting it is a practical method to establish CSA animal model.
观察在颈椎横突旁注射硬化剂诱导椎动脉型颈椎病（CSA）的效果。将20只家兔随机分为两组：模型组和对照组。模型组家兔在颈椎横突旁注射硬化剂，相反，对照组家兔不进行任何注射。采用经颅多普勒（TCD）检测椎动脉的血流平均速度（Vm）、搏动指数（Pi）和阻力指数（Ri），苏木精-伊红（HE）染色观察形态学变化，免疫组织化学检测α-平滑肌肌动蛋白（α-SMA）和基质金属蛋白酶-2（MMP-2）的表达。TCD显示，与对照组相比，模型组的Pi、Ri升高，Vm降低（P<0.05）。HE染色显示模型组平滑肌细胞增生肥大（P<0.05）。免疫组织化学显示模型组α-SMA和MMP-2表达上调（P<0.05）。结论是在颈椎横突旁注射硬化剂可诱导家兔椎动脉重塑，提示这是建立CSA动物模型的一种实用方法。
