Department of Medical Biochemistry, Medical University of Białystok, ul. Mickiewicza 2, 15-089 Białystok, Poland.
Mol Cell Biochem. 2010 Jul;340(1-2):81-9. doi: 10.1007/s11010-010-0403-z. Epub 2010 Feb 14.
Preeclampsia is the most common pregnancy-associated pathological syndrome. It is accompanied by the accumulation of free fatty acids, acylglycerols and cholesterol esters in the umbilical cord vein (UCV). We evaluate the sphingolipid composition of UCV and its alteration in preeclampsia. The veins were taken from 10 newborns delivered by healthy mothers and 10 newborns delivered by mothers with preeclampsia. Thin layer chromatography, solid-phase extraction and high-performance liquid chromatography were employed for sphingolipid analyses. The UCV walls of newborns delivered by healthy mothers are abundant in sphingomyelins and ceramides, whereas the amounts of sphingoid bases are rather low. Preeclampsia is associated with a significant decrease in sphingomyelins and ceramides, whereas the sphingoid bases changed in uncharacteristic manner. The increase in sphinganine and sphingosine 1-phosphate was accompanied with a decrease in sphingosine, hydroxysphinganine and sphinganine 1-phosphate. Stearate is the dominating fatty acid in sphingomyelins and ceramides of both control and preeclamptic veins. Sphingolipids and some sphingoid bases are bioactive molecules which contribute to regulation of signal transduction pathways, protein sorting and mediation of cell-to-cell interactions and recognition. The alteration in sphingolipid content may modify the metabolism of UCV wall resulting in remodelling of its composition.
子痫前期是最常见的妊娠相关病理综合征。它伴随着游离脂肪酸、酰基甘油和胆固醇酯在脐静脉 (UCV) 中的积累。我们评估了 UCV 的鞘脂组成及其在子痫前期中的变化。从 10 名由健康母亲分娩的新生儿和 10 名由患有子痫前期的母亲分娩的新生儿的静脉中采集样本。采用薄层色谱、固相萃取和高效液相色谱法进行鞘脂分析。健康母亲分娩的新生儿的 UCV 壁富含鞘磷脂和神经酰胺，而神经鞘氨醇的含量则相当低。子痫前期与鞘磷脂和神经酰胺的显著减少有关，而神经鞘氨醇则以特征性的方式发生变化。鞘氨醇、鞘氨醇 1-磷酸的增加伴随着鞘氨醇、羟基鞘氨醇和鞘氨醇 1-磷酸的减少。硬脂酸是控制和子痫前期静脉中鞘磷脂和神经酰胺的主要脂肪酸。鞘脂和一些神经鞘氨醇是生物活性分子，有助于调节信号转导途径、蛋白质分拣以及介导细胞间相互作用和识别。鞘脂含量的改变可能会改变 UCV 壁的代谢，从而改变其组成。
